Changes in Laminin Chain Expression in Pre- and Postnatal Rat Pituitary Gland

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Author(s)

    • Ramadhani Dini
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Tsukada Takehiro
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Fujiwara Ken
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Azuma Morio
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Kikuchi Motoshi
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine|Laboratory of Natural History, Jichi Medical University School of Medicine
    • Yashiro Takashi
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

Abstract

Cell–matrix interaction is required for tissue development. Laminin, a major constituent of the basement membrane, is important for structural support and as a ligand in tissue development. Laminin has 19 isoforms, which are determined by combinational assembly of five <font face="roman">α</font>, three <font face="roman">β</font>, and three <font face="roman">γ</font> chains (eg, laminin 121 is <font face="roman">α</font>1, <font face="roman">β</font>2, and <font face="roman">γ</font>1). However, no report has identified the laminin isoforms expressed during pituitary development. We used <i>in situ</i> hybridization to investigate all laminin chains expressed during rat anterior pituitary development. The <font face="roman">α</font>5 chain was expressed during early pituitary development (embryonic day 12.5–15.5). Expression of <font face="roman">α</font>1 and <font face="roman">α</font>4 chains was noted in vasculature cells at embryonic day 19.5, but later diminished. The <font face="roman">α</font>1 chain was re-expressed in parenchymal cells of anterior lobe from postnatal day 10 (P10), while the <font face="roman">α</font>4 chain was present in vasculature cells from P30. The <font face="roman">α</font>2 and <font face="roman">α</font>3 chains were transiently expressed in vasculature cells and anterior lobe, respectively, only at P30. Widespread distribution of <font face="roman">β</font> and <font face="roman">γ</font> chains was also observed during development. These findings suggest that numerous laminin isoforms are involved in anterior pituitary gland development and that alteration of the expression pattern is required for proper development of the gland.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 47(5), 231-237, 2014

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Codes

  • NII Article ID (NAID)
    130004700244
  • Text Lang
    ENG
  • ISSN
    0044-5991
  • Data Source
    J-STAGE 
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