ビタミンE欠乏食飼育マウス肝臓クッパー細胞内セロイド色素の組織化学的,電顕的研究

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  • Ultrastructural and Histochemical Studies on Ceroid Formed in the Kuffer Cell of the Liver from Vitam E Deficient Mice

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In 74 ICR/JCL mice aged 4 weeks (35 males and 39 females) fed on a vitamin E deficient diet for 55 weeks ceroid occurring in the liver tissues was observed at several stages of the experimental periods. Ceroid could be histochemically identified since 15 week feeding. Electron microscopical examination revealed swollen mitochondria and numerous autophagic vacuoles in the degenerated, atrophic liver cells consisting of dark cells and light cells.<BR>Some of dark cells phagocytized by Kupffer cells were seen discharged into sinu soid. Ceroid in the liver from mice fed on a vitamin E deficient diet appears to have been derived from complicated materials such as cell debris containing cellular membrane, degenerated mitochondria and autophagic vacuoles in the necrotizing liver cells.<BR>When observed in the electron microscope cero id formed in the liver from vitamin E deficient mice showed characteristic patterns such as band, lattice and parallel lamella pattern. Each band in the band pattern has a width measuring 45 to 60Åand each electron lucent space, a width measuring 70 to 90Å. The lattice pattern consists of three bands intersecting at an angle of 60 degrees forming a triangular space. Each band in the lattice pattern has a width similar to that of the band pattern.<BR>Parallel lamella pattern c onsists of lamellae of low electron density 90 to 120Åin width and each electron lucent space 45 to 55Åin width. From the above findings it has been eviden ced that ceroid appearing under the vitamin E deficient condition is electron microscopically characterized by the presence of the band, lattice and parallel lamella patterns.<BR>It is noted that n one of these patterns was found in ceroid formed in situ several weeks after insertion of a piece of liver tissues from untreated donor mice into the subcutaneous tissue of recipients.

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