Nanoscale Identification of Extracellular Organic Substances at the Microbe–Mineral Interface by Scanning Transmission X-ray Microscopy

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Author(s)

    • Mitsunobu Satoshi
    • Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka
    • Takahashi Yoshio
    • Institute of Materials Structure Science, High-Energy Accelerator Research Organization (KEK)|Department of Earth and Planetary Science, The University of Tokyo
    • Zhu Ming
    • Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka
    • Takeichi Yasuo
    • Institute of Materials Structure Science, High-Energy Accelerator Research Organization (KEK)|The Graduate University for Advanced Studies
    • Suga Hiroki
    • Department of Earth and Planetary Systems Science, Hiroshima University
    • Makita Hiroko
    • Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
    • Sakata Masahiro
    • Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka
    • Ono Kanta
    • Institute of Materials Structure Science, High-Energy Accelerator Research Organization (KEK)|The Graduate University for Advanced Studies
    • Mase Kazuhiko
    • Institute of Materials Structure Science, High-Energy Accelerator Research Organization (KEK)|The Graduate University for Advanced Studies

Abstract

In the present study, we applied a scanning transmission X-ray microscopy (STXM) technique to investigate the spatial distribution of biomolecules at the microbe–mineral boundary during bioleaching process of pyrite (FeS<sub>2</sub>). STXM-based carbon and oxygen near-edge X-ray absorption fine structure (NEXAFS) directly showed that polysaccharides were localized within 150 nm of the bacteria–pyrite boundary and that sessile bioleaching bacteria produced polysaccharides-abundant extracellular biomolecules on the pyrite grain. The STXM-based NEXAFS technique has multiple advantages: (i) a high spatial resolution less than 50 nm, (ii) a nondestructive analytical technique with a high element of specificity, (iii) X-ray spectroscopic chemical speciation useful for the analysis of hydrated biomolecules. Thus, the STXM-based NEXAFS technique is a powerful tool to investigate the mechanism of biological process occurring at the microbe–mineral interface.

Journal

  • Chemistry Letters

    Chemistry Letters 44(1), 91-93, 2015

    The Chemical Society of Japan

Codes

  • NII Article ID (NAID)
    130004701548
  • Text Lang
    ENG
  • ISSN
    0366-7022
  • Data Source
    J-STAGE 
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