Analysis of the Genetic Population Structure of <i>Cacopsylla chinensis</i> (Hemiptera: Psyllidae) with Mitochondrial DNA Markers in Saga and Yamaguchi prefectures, Japan

• KATOH Hiroshi

  NARO Institute of Fruit Tree Science

• INOUE Hiromitsu

  Kuchinotsu Citrus Research Station, NARO Institute of Fruit Tree Science

• UECHI Nami

  NARO Institute of Fruit Tree Science

• FUJIKAWA Takashi

  NARO Institute of Fruit Tree Science

• MIYATA Shin-ichi

  NARO Institute of Fruit Tree Science

• IWANAMI Toru

  NARO Institute of Fruit Tree Science

Bibliographic Information

Other Title

• Analysis of the Genetic Population Structure of Cacopsylla chinensis (Hemiptera: Psyllidae) with Mitochondrial DNA Markers in Saga and Yamaguchi prefectures, Japan

Abstract

DNA sequence analysis of the mitochondrial cytochrome oxidase I (COI)-leucine tRNA (tRNA^Leu)-cytochrome oxidase II (COII) regions was performed to elucidate the phylogenetic relationships among 465 summer and winter-form psyllids from Saga and Yamaguchi prefectures in Japan. Multiple alignments of these sequences revealed 2 single-nucleotide polymorphisms (SNPs) at nucleotide positions 78 and 258 in the COI and tRNA^Leu regions, respectively. Based on the nucleotide changes at these loci, these 465 insects were grouped into 3 genotypes: 78A/258A, 78G/258G, and 78G/258A. All but 2 of the 225 insects in Saga belonged to the genotype 78A/258A, and the 2 exceptions belonged to the genotype 78G/258G. Conversely, all the insects (105) collected from Shimonoseki city in western Yamaguchi prefecture belonged to the genotype 78G/258G, while eighty percent of the 135 samples from eastern Yamaguchi prefecture belonged to the genotype 78G/258G. Among the other 27 insect samples, 24 were 78G/258A and 3 were 78A/258A. Using POPTREE2, the psyllids were divided into 5 populations associated with the geographical distribution. The results suggest that the genetic population structure of these psyllids varied widely between Saga and Yamaguchi prefectures.

Journal

• Japan Agricultural Research Quarterly: JARQ

  Japan Agricultural Research Quarterly: JARQ 48 (4), 413-417, 2014

  Japan International Research Center for Agricultural Sciences

Keywords

• cytochrome oxidase I
• molecular phylogenetic analysis
• Pear psyllids
• population genetics

Details

• CRID: 1390001204701817472
• NII Article ID: 130004701844
• DOI: 10.6090/jarq.48.413
• ISSN: 21858896
• ISSN: 00213551
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed