光線力学的療法 (PDT) による白血病細胞株P388に対する細胞障害性 Cytotoxicity of Photodynamic Therapy (PDT) for P388 Leukemia cells
The cytotoxicity of PDT with ME2906 and Laser to P388 cells was examined. Cell viability was assayed by the MTT method. When the laser irradiated at 10J/cm<SUP>2</SUP> after incubation for 4 hours with 10μg/ml of ME2906, cell viability was not decreased but with 20 and 30μg/ml of ME2906, cell viabilities were 9 and 1.4% respectively. When the laser irradiated at 10J/cm<SUP>2</SUP> after incubation for 24 hours, cell viabilities were almost same as those of 4 hours incubation. ME2906 contents at 24 hours after drug administration at concentration of 10μg/ml measured 2.8×10<SUP>-9</SUP>μg/cell, and at 4 hours after drug administration at 20μg/ml measured 5.5×10<SUP>-9</SUP>μg/cell. These data showed that the cytotoxicity of PDT to P388 cells was dependent on the ME2906 intracellular accumulated contents but independent on laser doses.
日本レーザー医学会誌 17(Supplement), 281-284, 1996