TFE3 Is a bHLH-ZIP-type Transcription Factor that Regulates the Mammalian Golgi Stress Response

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Author(s)

    • Taniguchi Mai
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Matsumura Yasuyo
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Fujita Natsumi
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Araki Naoko
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Yamamoto Mayu
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Oku Masaya
    • Department of Biophysics, Graduate School of Science, Kyoto University
    • Wakabayashi Sadao
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Yoshida Hiderou
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo|Department of Biophysics, Graduate School of Science, Kyoto University
    • Tanakura Soichiro
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Sawaguchi Shogo
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Midori Sachiko
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Kawai Yumeto
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Yamaguchi Shogo
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Shimada Yui
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
    • Nakamura Yuki
    • Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo

Abstract

The Golgi stress response is a mechanism by which, under conditions of insufficient Golgi function (Golgi stress), the transcription of Golgi-related genes is upregulated through an enhancer, the Golgi apparatus stress response element (GASE), in order to maintain homeostasis in the Golgi. The molecular mechanisms associated with GASE remain to be clarified. Here, we identified TFE3 as a GASE-binding transcription factor. TFE3 was phosphorylated and retained in the cytoplasm in normal growth conditions, whereas it was dephosphorylated, translocated to the nucleus and activated Golgi-related genes through GASE under conditions of Golgi stress, e.g. in response to inhibition of oligosaccharide processing in the Golgi apparatus. From these observations, we concluded that the TFE3-GASE pathway is one of the regulatory pathways of the mammalian Golgi stress response, which regulates the expression of glycosylation-related proteins in response to insufficiency of glycosylation in the Golgi apparatus.

Journal

  • Cell Structure and Function

    Cell Structure and Function 40(1), 13-30, 2015

    Japan Society for Cell Biology

Codes

  • NII Article ID (NAID)
    130004703720
  • Text Lang
    ENG
  • ISSN
    0386-7196
  • Data Source
    J-STAGE 
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