Development and characterization of 27 new microsatellite markers for the Indian bullfrog Hoplobatrachus tigerinus and its congeneric species
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- Sultana Nasrin
- Institute for Amphibian Biology, Graduate School of Science, Hiroshima University
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- Igawa Takeshi
- Institute for Amphibian Biology, Graduate School of Science, Hiroshima University Division of Development Science, Graduate School of International Development and Cooperation, Hiroshima University
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- Nozawa Masafumi
- Center for Information Biology, National Institute of Genetics Department of Genetics, The Graduate University for Advanced Studies (Sokendai)
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- Islam Mohammed Mafizul
- Institute for Amphibian Biology, Graduate School of Science, Hiroshima University
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- Hasan Mahmudul
- Institute for Amphibian Biology, Graduate School of Science, Hiroshima University
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- Alam Mohammad Shafiqul
- Department of Genetics and Fish Breeding, Faculty of Fisheries, Bangabandhu Sheikh Mujibur Rahman Agricultural University
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- Khan Md. Mukhlesur Rahman
- Department of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University
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- Sumida Masayuki
- Institute for Amphibian Biology, Graduate School of Science, Hiroshima University
書誌事項
- タイトル別名
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- Development and characterization of 27 new microsatellite markers for the Indian bullfrog <i>Hoplobatrachus tigerinus</i> and its congeneric species
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抄録
The Indian bullfrog Hoplobatrachus tigerinus and its four congeneric species are common frog species and distributed throughout South Asia. Due to recent human activity, they are facing a changing environment and reduction in natural population size. For effective conservation and molecular ecological studies, we therefore isolated and characterized microsatellite loci for these frogs. We obtained genomic data using an Ion Torrent PGM sequencer and designed 54 primer sets for candidate loci. By screening for polymorphic loci in individuals of H. tigerinus and its congeneric species, we isolated 27 loci as highly polymorphic microsatellite loci. Eight of these loci were commonly applicable for all species except H. chinensis. Within two populations of H. tigerinus, the total number of alleles per locus and expected heterozygosity ranged from 2 to 18 and 0.271 to 0.938, respectively. No significant linkage disequilibrium was observed across all loci, and five showed a significant deviation from Hardy-Weinberg equilibrium in some populations after Bonferroni correction. Consequently, our findings suggest that these novel markers will be applicable for conservation genetic studies across varying scales from inter-population to inter-individual.
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 89 (3), 137-141, 2014
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390001205472600448
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- NII論文ID
- 130004703732
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 025931983
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- PubMed
- 25475937
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可