A Fine-Scale Phylogenetic Analysis of Free-Living <i>Burkholderia</i> Species in Sugarcane Field Soil
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The diversity and abundance of <i>Burkholderia</i> species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus <i>Burkholderia</i>. Nearly 58% of these sequences were placed in a previously defined cluster, including stinkbug symbionts. Quantitative PCR analysis revealed a consistent number of 16S rRNA gene copies for <i>Burkholderia</i> species (10<sup>7</sup> g<sup>−1</sup> soil) across the sampled fields. C/N, pH, and nitrate concentrations were important factors shaping the <i>Burkholderia</i> community structure; however, their impacts were not significant considering the overall genus size.
- Microbes and Environments
Microbes and Environments 29(4), 434-437, 2014
Japanese Society of Microbial Ecology · The Japanese Society of Soil Microbiology