A Fine-Scale Phylogenetic Analysis of Free-Living <i>Burkholderia</i> Species in Sugarcane Field Soil

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Author(s)

    • Tago Kanako
    • Environmental Biofunction Division, National Institute for Agro–Environmental Sciences
    • Hayashi Kentaro
    • Environmental Biofunction Division, National Institute for Agro–Environmental Sciences
    • Hayatsu Masahito
    • Environmental Biofunction Division, National Institute for Agro–Environmental Sciences
    • Itoh Hideomi
    • Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Hokkaido
    • Kikuchi Yoshitomo
    • Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Hokkaido
    • Hori Tomoyuki
    • Research Institute for Environmental Management Technology, AIST
    • Sato Yuya
    • Research Institute for Environmental Management Technology, AIST
    • Okubo Takashi
    • Environmental Biofunction Division, National Institute for Agro–Environmental Sciences
    • Navarro Ronald
    • Research Institute for Environmental Management Technology, AIST
    • Aoyagi Tomo
    • Research Institute for Environmental Management Technology, AIST

Abstract

The diversity and abundance of <i>Burkholderia</i> species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus <i>Burkholderia</i>. Nearly 58% of these sequences were placed in a previously defined cluster, including stinkbug symbionts. Quantitative PCR analysis revealed a consistent number of 16S rRNA gene copies for <i>Burkholderia</i> species (10<sup>7</sup> g<sup>−1</sup> soil) across the sampled fields. C/N, pH, and nitrate concentrations were important factors shaping the <i>Burkholderia</i> community structure; however, their impacts were not significant considering the overall genus size.

Journal

  • Microbes and Environments

    Microbes and Environments 29(4), 434-437, 2014

    Japanese Society of Microbial Ecology · The Japanese Society of Soil Microbiology

Codes

  • NII Article ID (NAID)
    130004703873
  • Text Lang
    ENG
  • ISSN
    1342-6311
  • Data Source
    J-STAGE 
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