A Fine-Scale Phylogenetic Analysis of Free-Living <i>Burkholderia</i> Species in Sugarcane Field Soil

• Tago Kanako

  Environmental Biofunction Division, National Institute for Agro–Environmental Sciences

• Itoh Hideomi

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Hokkaido

• Kikuchi Yoshitomo

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Hokkaido

• Hori Tomoyuki

  Research Institute for Environmental Management Technology, AIST

• Sato Yuya

  Research Institute for Environmental Management Technology, AIST

• Nagayama Atsushi

  Okinawa Prefectural Agricultural Research Center

• Okubo Takashi

  Environmental Biofunction Division, National Institute for Agro–Environmental Sciences

• Navarro Ronald

  Research Institute for Environmental Management Technology, AIST

• Aoyagi Tomo

  Research Institute for Environmental Management Technology, AIST

• Hayashi Kentaro

  Environmental Biofunction Division, National Institute for Agro–Environmental Sciences

• Hayatsu Masahito

  Environmental Biofunction Division, National Institute for Agro–Environmental Sciences

Bibliographic Information

Other Title

• A Fine-Scale Phylogenetic Analysis of Free-Living Burkholderia Species in Sugarcane Field Soil

Abstract

The diversity and abundance of Burkholderia species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus Burkholderia. Nearly 58% of these sequences were placed in a previously defined cluster, including stinkbug symbionts. Quantitative PCR analysis revealed a consistent number of 16S rRNA gene copies for Burkholderia species (10⁷ g⁻¹ soil) across the sampled fields. C/N, pH, and nitrate concentrations were important factors shaping the Burkholderia community structure; however, their impacts were not significant considering the overall genus size.

Journal

• Microbes and Environments

  Microbes and Environments 29 (4), 434-437, 2014

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• <i>Burkholderia</i>
• Illumina sequencing
• sugarcane field

Details

• CRID: 1390001204345608576
• NII Article ID: 40020304084
• NII Article ID: 130004703873
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me14122
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 025987380
• PubMed: 25410730
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025987380
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed