Nutrient starvation affects expression of LC3 family at the feto-maternal interface during murine placentation

• HIYAMA Masato

  Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan

• KUSAKABE Ken Takeshi

  Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan Laboratory of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753–8515, Japan

• TAKESHITA Ai

  Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Science, Osaka Prefecture University, Osaka 598–8531, Japan

• SUGI Sinji

  Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan

• KUNIYOSHI Nobue

  Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan

• IMAI Hiroyuki

  Laboratory of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753–8515, Japan

• KANO Kiyoshi

  Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan Laboratory of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753–8515, Japan

• KISO Yasuo

  Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan Laboratory of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753–8515, Japan

Abstract

LC3 − the mammalian homolog of Atg8 − was found as autophagosome membrane binding protein in mammals and widely used as an autophagosomal marker. LC3A, B and C show different expression patterns in each tissue. The aim of this study was to reveal the differences of expression patterns among LC3 families in mouse placenta under normal condition and nutrient starving condition. LC3A and B were highly expressed in decidual cells. LC3A and B were increased in D14 compared with D12 and D16 in mouse placenta, while LC3C was decreased. Starvation induced increase in LC3B expression specifically. Immunohistochemistry showed different expression patterns among LC3A, B and C. LC3A expression in syncytiotrophoblast was vanished by starvation. The results of real time RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by starvation. Taken together, this study suggests that autophagy could play a role in placental invasion system and that nutrient starvation affects LC3B expression.

Journal

• Journal of Veterinary Medical Science

  Journal of Veterinary Medical Science 77 (3), 305-311, 2015

  JAPANESE SOCIETY OF VETERINARY SCIENCE

Keywords

• autophagy
• LC3
• mTOR
• placenta
• starvation

Details

• CRID: 1390001206429246720
• NII Article ID: 130004704141
• NII Book ID: AA10796138
• DOI: 10.1292/jvms.14-0490
• ISSN: 13477439
• ISSN: 09167250
• NDL BIB ID: 026282001
• PubMed: 25421500
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026282001
• Web Site: https://search.jamas.or.jp/link/ui/2015281148
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed