Carbon Nanofiber-based Luminol-biotin Probe for Sensitive Chemiluminescence Detection of Protein

• BAJ Stefan

  Department of Chemical Organic Technology and Petrochemistry, Faculty of Chemistry, Silesian University of Technology

• KRAWCZYK Tomasz

  Department of Chemical Organic Technology and Petrochemistry, Faculty of Chemistry, Silesian University of Technology

• PRADEL Natalia

  Department of Chemical Organic Technology and Petrochemistry, Faculty of Chemistry, Silesian University of Technology

• AZAM Md. Golam

  Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University

• SHIBATA Takayuki

  Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University

• DRAGUSHA Shpend

  Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University

• SKUTIL Krzysztof

  Department of Chemical Organic Technology and Petrochemistry, Faculty of Chemistry, Silesian University of Technology

• PAWLYTA Miroslawa

  Institute of Engineering Materials and Biomaterials, Silesian University of Technology

• KAI Masaaki

  Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University

Abstract

A carbon nanofiber-based luminol-biotin probe was synthesized for the sensitive chemiluminescence (CL) detection of a target protein by grafting luminol and biotin onto an oxidized carbon nanofiber. This carbon nanofiber was prepared by chemical vapor-deposition with methane in the presence of the Ni–Cu–MgO catalyst, which was followed by oxidization with HNO₃–H₂SO₄ to produce a carboxyl group on the surface of the nanofiber. The material was grafted with luminol and biotin by means of a standard carbodiimide activation of COOH groups to produce corresponding amides. The substance was water-soluble and thus could be utilized as a sensitive CL probe for a protein assay. The probe showed highly specific affinity towards the biotin-labeled antibody via a streptavidin–biotin interaction. The detection limit for this model assay was approximately 0.2 pmol of the biotinized IgG spotted on a polyvinylidene fluoride (PVDF) membrane. Nonspecific binding to other proteins was not observed. Therefore, the synthesized carbon nanofiber-based CL probe may be useful for a sensitive and specific analysis of the target protein.

Journal

• Analytical Sciences

  Analytical Sciences 30 (11), 1051-1056, 2014

  The Japan Society for Analytical Chemistry

Keywords

• Chemiluminescence
• carbon nanofiber
• luminol
• proteins
• streptavidin

Details

• CRID: 1390282679236939648
• NII Article ID: 130004704337
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.30.1051
• ISSN: 13482246
• ISSN: 09106340
• HANDLE: 10069/35280
• NDL BIB ID: 025902562
• PubMed: 25382040
• Web Site: https://nagasaki-u.repo.nii.ac.jp/records/4166
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025902562
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.30.1051.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.30.1051/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/30/11/30_1051/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015136892
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed