抗血液型抗体による血液型抗原中和療法
書誌事項
- タイトル別名
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- Neutralization of blood group A-antigen by anti-A antibody
抄録
Background. The major barrier to ABO-incompatible solid organ transplantation is acute humoral rejection. Various strategies to reduce anti-blood group antibodybyovercoming ABO incompatible transplantation have been tried. However, antigen-suppressing procedures have not been performed. Methods. We investigated whether an anti-A antibody(K7508)can mask A-antigen on the cells in vitro. Next, we immunized mice with A-antigen-expressing cells coated with K7508 or its Fab fragment, and measured anti-A antibodyproduction in the mice. Results. Blood group A-antigen-expressing cells coated with K7508 were not recognized by another anti-A antibodyin flow cytometry, indicating that A-antigen was masked byK7508 in vitro. The A-antigen on the paraffin-embedded liver tissue was also masked byK7508. Furthermore, the production of anti-A antibodyin mice immunized with A-antigen-expressing cells coated with K7508 or its Fab fragment was significantlysuppressed compared to that in mice immunized with non-coated cells alone, indicating that A-antigen was neutralized byK7508 in vivo. Conclusions. The neutralization of blood group antigen byanti-blood group antibodyand especiallyits Fab fragment might represent one strategyto overcome ABO-incompatible organ transplantation
収録刊行物
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- Organ Biology
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Organ Biology 18 (1), 112-115, 2011
一般社団法人 日本臓器保存生物医学会
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詳細情報 詳細情報について
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- CRID
- 1390282680490855552
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- NII論文ID
- 130004709992
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- COI
- 1:CAS:528:DC%2BC3MXhtFSgsL%2FN
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- ISSN
- 21880204
- 13405152
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可