Immunohistochemical Characteristics of Surfactant Proteins A, B, C and D in Inflammatory and Tumorigenic Lung Lesions of F344 Rats

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Author(s)

    • Yamakawa Keiko
    • Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
    • Nakano Yuko
    • Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
    • Kishi Sosuke
    • Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
    • Ninomiya Fumiko
    • Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
    • Kanie Shohei
    • Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
    • Hitotsumachi Hiroko
    • Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University

Abstract

Surfactant proteins (SPs), originally known as human lung surfactants, are essential to respiratory structure and function. There are 4 subtypes, SP-A, SP-B, SP-C and SP-D, with SP-A and SP-D having immunological functions, and SP-B and SP-C having physicochemical properties that reduce the surface tension at biological interfaces. In this experiment, the expressions of SP-A, SP-B, SP-C and SP-D in lung neoplastic lesions induced by N-bis (2-hydroxypropyl) nitrosamine (DHPN) and inflammatory lesions due to quartz instillation were examined and compared immunohistochemically. Formalin fixed paraffin embedded (FFPE) lung samples featuring inflammation were obtained with a rat quartz instillation model, and neoplastic lesions, hyperplasias and adenomas, were obtained with the rat DHPN-induced lung carcinogenesis model. In the rat quartz instillation model, male 10-week old F344 rats were exposed by intratracheal instillation (IT) to quartz at a dose of 2 mg/rat suspended in saline (0.2 ml) on day 0, and sacrificed on day 28. Lung tumorigenesis in F344 male rats was initiated by DHPN in drinking water for 2 weeks, and the animals were then sacrificed in week 30. Lung proliferative lesions, hyperplasias and adenomas, were observed with DHPN, and inflammation was observed with quartz. The expressions of SP-A, SP-B, SP-C and SP-D were examined immunohistochemically. SP-B and SP-C showed strong expression in lung hyperplasias and adenomas, while SP-A and SP-D were observed in mucus or exudates in inflammatory alveoli. These results suggest the possibility that SP-B and SP-C are related to lung tumorigenesis.

Journal

  • Journal of Toxicologic Pathology

    Journal of Toxicologic Pathology 27(3+4), 175-182, 2014

    JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY

Codes

  • NII Article ID (NAID)
    130004713012
  • NII NACSIS-CAT ID (NCID)
    AN10232280
  • Text Lang
    ENG
  • ISSN
    0914-9198
  • NDL Article ID
    025899768
  • NDL Call No.
    Z19-2431
  • Data Source
    NDL  J-STAGE 
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