Immunohistochemical Characteristics of Surfactant Proteins A, B, C and D in Inflammatory and Tumorigenic Lung Lesions of F344 Rats
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- Yokohira Masanao
- Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
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- Yamakawa Keiko
- Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
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- Nakano Yuko
- Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
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- Numano Takamasa
- DIMS Institute of Medical Science, Inc.
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- Furukawa Fumio
- DIMS Institute of Medical Science, Inc.
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- Kishi Sosuke
- Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
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- Ninomiya Fumiko
- Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
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- Kanie Shohei
- Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
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- Hitotsumachi Hiroko
- Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
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- Saoo Kousuke
- Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
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- Imaida Katsumi
- Onco-Pathology, Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University
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Abstract
Surfactant proteins (SPs), originally known as human lung surfactants, are essential to respiratory structure and function. There are 4 subtypes, SP-A, SP-B, SP-C and SP-D, with SP-A and SP-D having immunological functions, and SP-B and SP-C having physicochemical properties that reduce the surface tension at biological interfaces. In this experiment, the expressions of SP-A, SP-B, SP-C and SP-D in lung neoplastic lesions induced by N-bis (2-hydroxypropyl) nitrosamine (DHPN) and inflammatory lesions due to quartz instillation were examined and compared immunohistochemically. Formalin fixed paraffin embedded (FFPE) lung samples featuring inflammation were obtained with a rat quartz instillation model, and neoplastic lesions, hyperplasias and adenomas, were obtained with the rat DHPN-induced lung carcinogenesis model. In the rat quartz instillation model, male 10-week old F344 rats were exposed by intratracheal instillation (IT) to quartz at a dose of 2 mg/rat suspended in saline (0.2 ml) on day 0, and sacrificed on day 28. Lung tumorigenesis in F344 male rats was initiated by DHPN in drinking water for 2 weeks, and the animals were then sacrificed in week 30. Lung proliferative lesions, hyperplasias and adenomas, were observed with DHPN, and inflammation was observed with quartz. The expressions of SP-A, SP-B, SP-C and SP-D were examined immunohistochemically. SP-B and SP-C showed strong expression in lung hyperplasias and adenomas, while SP-A and SP-D were observed in mucus or exudates in inflammatory alveoli. These results suggest the possibility that SP-B and SP-C are related to lung tumorigenesis.
Journal
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- Journal of Toxicologic Pathology
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Journal of Toxicologic Pathology 27 (3+4), 175-182, 2014
JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY
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Details 詳細情報について
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- CRID
- 1390282679392887936
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- NII Article ID
- 130004713012
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- NII Book ID
- AN10232280
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- ISSN
- 1881915X
- 09149198
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- NDL BIB ID
- 025899768
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- PubMed
- 25378802
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed