Regulation of xylanase elicitor-induced expression of defense-related genes involved in phytoalexin biosynthesis by a cation channel OsTPC1 in suspension-cultured rice cells

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Author(s)

    • Hamada Haruyasu
    • Department of Applied Biological Science, Tokyo University of Science
    • Kurusu Takamitsu
    • Department of Applied Biological Science, Tokyo University of Science|School of Bioscience and Biotechnology, Tokyo University of Technology
    • Kuchitsu Kazuyuki
    • Department of Applied Biological Science, Tokyo University of Science|Research Institute for Science and Technology, 
Tokyo University of Science

Abstract

Signal molecules derived from pathogens/microbes or plants (pathogen/microbe/damage-associated molecular patterns; PAMPs/MAMPs/DAMPs), elicitors, trigger changes in cytosolic free Ca<sup>2+</sup> concentrations ([Ca<sup>2+</sup>]<sub>cyt</sub>) to activate plant immune responses. A rice two-pore channel 1 (OsTPC1) has been suggested to be involved in fungal xylanase elicitor (TvX)-induced defense responses including [Ca<sup>2+</sup>]<sub>cyt</sub> increase, phytoalexin production and hypersensitive cell death in suspension-cultured rice cells. However, little is known on the molecular links between [Ca<sup>2+</sup>]<sub>cyt</sub> rise and elicitor-induced gene expression. To gain insights on the possible roles of OsTPC1 in TvX-induced gene expression, we performed DNA microarray analysis using a rice 44K oligo-microarray system, and revealed that TvX induce expression of thousands of genes including WRKY-type transcription factors, a serine hydrolase involved in hypersensitive cell death, and diterpene cyclases required for phytoalexin biosynthesis, which are suppressed in the <i>Ostpc1</i> knockout mutant. TvX-induced expression of genes involved in the methylerythritol phosphate (MEP) pathway, which is located upstream of the phytoalexin biosynthesis pathway, was also suppressed in <i>Ostpc1</i> cells. Possible involvement of OsTPC1 in the regulation of gene expression and metabolism in cultured-rice cells is discussed.

Journal

  • Plant Biotechnology

    Plant Biotechnology 31(4), 329-334, 2014

    Japanese Society for Plant Cell and Molecular Biology

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