Metabolic engineering of flavonoids with prenyltransferase and chalcone isomerase genes in tomato fruits
Access this Article
Flavonoids are phenolic secondary metabolites commonly occurring in plants. In particular, prenylated flavonoids exhibit a wide range of biological activities, including antitumor, antibacterial and antioxidant activities. In this study, we attempted to produce prenylated flavonoids in tomato as a host plant by means of metabolic engineering through the introduction of both the naringenin 8-dimethylallyltransferase (<i>N8DT</i>) gene, encoding a prenyltransferase from <i>Sophora flavescens</i>, and the chalcone isomerase (<i>CHI</i>) gene from <i>Nicotiana tabacum</i> cv. Samsun-NN. Liquid chromatography-tandem mass spectrometry analysis revealed the production of 8-dimethylallyl naringenin in the double transformants, while the production level was lower than N8DT single transformants. In addition, tomato fruits over-expressing both <i>N8DT</i> and <i>CHI</i> genes accumulated high levels of rutin compared with wild-type tomato. A possible endogenous regulation of the synthesis of flavonoid derivatives is discussed.
- Plant Biotechnology
Plant Biotechnology 31(5), 567-571, 2014
Japanese Society for Plant Cell and Molecular Biology