Ultrasonication-Induced Acceleration of Amyloid Fibrillation and Protein Crystallization

• SO Masatomo

  Institute for Protein Research, Osaka University

• YAGI Hisashi

  Center for Research on Green Sustainable Chemistry, Tottori University

• GOTO Yuji

  Institute for Protein Research, Osaka University

Bibliographic Information

Other Title

• 超音波による蛋白質のアミロイド形成・結晶化の促進
• チョウオンパ ニ ヨル タンパクシツ ノ アミロイド ケイセイ ・ ケッショウカ ノ ソクシン

Abstract

Protein crystals form in supersaturated solutions via a nucleation and growth mechanism. The amyloid fibrils of denatured proteins also form via a nucleation and growth mechanism. This similarity suggests that, although protein crystals and amyloid fibrils are distinct in their morphologies, both processes can be controlled in a similar manner. We show that ultrasonication is one of the most effective methods of agitation for accelerating spontaneous fibrillation. We also show that ultrasonication is also useful for forming crystals of proteins. We address the underlying mechanism of the ultrasonication-dependent breakdown of supersaturation, leading to the formation of amyloid fibrils or protein crystals.

Journal

• Seibutsu Butsuri

  Seibutsu Butsuri 54 (6), 297-302, 2014

  The Biophysical Society of Japan General Incorporated Association

Keywords

• amyloid fibrillation
• crystallization
• solubility
• supersaturation
• ultrasonication

Details

• CRID: 1390001206534956928
• NII Article ID: 130004713028
• NII Book ID: AN00129693
• DOI: 10.2142/biophys.54.297
• ISSN: 13474219
• ISSN: 05824052
• NDL BIB ID: 025953742
• Web Site: http://id.ndl.go.jp/bib/025953742
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025953742
• Web Site: https://www.jstage.jst.go.jp/article/biophys/54/6/54_297/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015092290
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed