Comparison of platelet serotonin transporter activity in subjects with severe sleep bruxism and control

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Author(s)

    • Minakuchi Hajime
    • Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Kuboki Takuo
    • Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Sogawa Chiharu
    • Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Hara Emilio Satoshi
    • Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Miki Haruna
    • Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Maekawa Kenji
    • Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Sogawa Norio
    • Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Kitayama Shigeo
    • Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
    • Matsuka Yoshizo
    • Department of Fixed Prosthodontics, Institute of Health Biosciences Graduate School, The University of Tokushima
    • Clark Glenn T.
    • Advanced Program in Orofacial Pain and Oral Medicine, Ostrow School of Dentistry, University of Southern California

Abstract

<i>Purpose:</i> The aim of this study was to evaluate the correlation between sleep bruxism (SB) frequency and serotonin transporter (SERT)-driven serotonin (5-HT)-uptake in platelets.<br><i>Methods:</i> Subjects were dental trainee residents and faculty members of Okayama University Hospital who were aware of having severe or no SB. SB frequency was assessed for 3-consecutive nights by a self-contained electromyographic detector/analyzer, which indicated individual SB levels as one of four grades (score 0, 1, 2 and 3). Subjects were classified as normal control (NC) when SB scores indicated only 0 or 1 during the 3 nights, or as severe SB for scores 2 or 3. Those subjects whose scores fluctuated from 0 to 3 during the 3 nights were omitted from further analysis. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. Amounts of SERTs proteins collected from peripheral platelets were quantified using ELISA, and SERTs transport activity was assessed by uptake assay using [<ce:sup loc="pre">3</sup>H]-5-HT.<br><i>Results:</i> Thirteen severe SB subjects and 7 NC subjects were eligible. Gender distribution, mean age, 5-HT concentration and total amounts of SERT protein in platelets showed no significant differences between NC and severe SB (<i>p</i> = 0.85: <i>Chi-squared test</i>; <i>p</i> = 0.64, 0.26, 0.46: <i>t-test</i>). However, [<ce:sup loc="pre">3</sup>H]-5-HT uptake by platelets was significantly greater in NC compared to severe SB subjects (12.79 ± 1.97, 8.27 ± 1.91 fmol/10<sup>5</sup> platelets/min, <i>p</i> < 0.001, <i>t-test</i>).<br><i>Conclusion:</i> The results of this pilot study suggest a possible correlation between peripheral platelet serotonin transporter uptake ability and SB severity.

Journal

  • Journal of Prosthodontic Research

    Journal of Prosthodontic Research 58(4), 217-222, 2014

    Japan Prosthodontic Society

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