Production of C-glucosides of flavonoids and related compounds by Escherichia coli expressing buckwheat C-glucosyltransferase
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- Ito Takamitsu
- Faculty of Textile Science and Technology, Shinshu University
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- Fujimoto Shunsuke
- Faculty of Textile Science and Technology, Shinshu University
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- Shimosaka Makoto
- Faculty of Textile Science and Technology, Shinshu University
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- Taguchi Goro
- Faculty of Textile Science and Technology, Shinshu University
書誌事項
- タイトル別名
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- Production of <i>C</i>-glucosides of flavonoids and related compounds by <i>Escherichia coli</i> expressing buckwheat <i>C</i>-glucosyltransferase
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抄録
C-Glucosides are glucose-containing glycosides that have carbon–carbon bonds between the anomeric carbon of the sugar moieties and aglycon, rendering the molecules remarkably stable against hydrolysis by enzymes or acids. In this work, we showed the production of C-glucosides of flavonoids and related compounds (i.e., 2-hydroxyflavanone, dihydrochalcone, and trihydroxyacetophenone) by Escherichia coli expressing buckwheat C-glucosyltransferase. The substrates in their respective cultures were taken up by the cells and C-glucosylated, and the products were released into the culture media. The bioconversion process was completed in 1–2 h, but products were already observed immediately after addition of the substrates (200 µM). The conversion rates of these substrates reached 80–95%. Without addition of glucose to the conversion media, almost no C-glucosides were produced. Although the amounts of the substrates fed to their respective cultures were limited by their solubility in water, repeated addition of the substrate to the culture at regular time intervals effectively increased the total amount of product obtained.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 31 (5), 519-524, 2014
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204326847744
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- NII論文ID
- 130004721170
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- HANDLE
- 10091/0002001060
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- NDL書誌ID
- 026092163
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可