Expression of TRAF6 mRNA on the Resorbed Surface of Deciduous Teeth Root
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- Zhang Guirong
- Department of Orthodontics, Stomatology Hospital of Shenyang
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- Nakano Keisuke
- Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine
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- Honda Yuma
- Center for Advanced Oral Science, Aichi Gakuin University Department of Oral Pathology, School of Dentistry, Aichi Gakuin University
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- Wang Xue
- Department of Orthodontics, Stomatology Hospital of Shenyang
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- Wang Minghua
- Department of Orthodontics, Stomatology Hospital of Shenyang
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- Xu Yan
- Department of Orthodontics, Stomatology Hospital of Shenyang
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- Kawakami Toshiyuki
- Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine
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Abstract
The replacement of deciduous teeth by permanent teeth was an in vivo controlled physiological course. Odontoclast is the main functional cell involved the physiological root resorption of human primary teeth. TRAF6 is the key molecule in the signals regulation during the procedure of differentiation and maturity of osteolasts/odontoclasts. This experiment was to study the expression of TRAF6 in physiological root resorption of deciduous and permanent teeth. In addition, the effect of TRAF6 during the physiological root resorption of human deciduous teeth was also discussed. Permanent and deciduous teeth in physiological resorption period were decalcified, embedded and sliced. TRAF6 antibody and TRAF6 in situ hybridization agent were used to detect samples’ expression of TRAF6 protein and mRNA. Im-pro-plas 6.0 analytical system was used to measure the photodensity of odontoclasts, odontoblasts and dental pulp fibroblasts respectively between study and control group. It was observed that TRAF6 protein and mRNA of odontoclasts, odontoblasts and dental pulp fibroblasts existed in deciduous teeth with physiological resorption showing positive stained results. However, in permanent tooth, odontoclast was not observed and TRAF6 in odontoblast and dental pulp fibroblasts displayed negative. For the same cells of the two groups, t-test showed that p‹0.01. The optical density of the deciduous teeth odontoclasts was higher than that of the odontoblasts and dental pulp fibroblasts, P‹0.01; the odontoblasts and dental pulp fibroblasts showed no difference. TRAF6 activated the root resorption of deciduous teeth by participating in cellular differentiation and function.
Journal
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 20 (3), 177-184, 2011
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
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Details 詳細情報について
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- CRID
- 1390001204457267968
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- NII Article ID
- 130004722017
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- ISSN
- 1880828X
- 13417649
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed