Period is involved in the proliferation of human pancreatic MIA-PaCa2 cancer cells by TNF-.ALPHA.

• SUZUKI Takahiro

  Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine

• SATO Fuyuki

  Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine

• KONDO Jun

  Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine

• LIU Yang

  Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine

• KUSUMI Tomomi

  Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine

• FUJIMOTO Katsumi

  Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences

• KATO Yukio

  Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences

• SATO Tatsusuke

  Department of Pathologic Analysis, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences

• KIJIMA Hiroshi

  Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine

Bibliographic Information

Other Title

• Period is involved in the proliferation of human pancreatic MIA-PaCa2 cancer cells by TNF-α

Abstract

Recent studies have found alterations to clock genes in several forms of cancer. Period (Per) gene plays an important role in the circadian system, the cell cycle, the induction of apoptosis, and DNA damage. However, the functions of Per in pancreatic cancer have not yet been elucidated. Here, we show that tumor necrosis factor-α (TNF-α) suppressed the expression of both Per1 and Per3 in MIA-PaCa2 cells, a human pancreatic cancer cell line. The levels of these proteins were 10% lower in the cells treated with 10 ng/mL TNF-α. Cell proliferation showed a 15%, 14%, and 16% decrease at 0.4, 2.0, and 10 ng/mL of TNF-α, respectively. In MTS-assays, MIA-PaCa2 cells transfected with siRNA against Per1 showed a 19% reduction in proliferation. However, the knockdown of Per3 did not significantly inhibit cell proliferation. The results suggest Per1 to be involved in the inhibition of the proliferation of MIA-PaCa2 cells by TNF-α.

Journal

• Biomedical Research

  Biomedical Research 29 (2), 99-103, 2008

  Biomedical Research Press

Keywords

• General Biochemistry, Genetics and Molecular Biology
• General Medicine

Details

• CRID: 1390001204900062592
• NII Article ID: 130004724055
• DOI: 10.2220/biomedres.29.99
• ISSN: 1880313X
• ISSN: 03886107
• Web Site: https://search.jamas.or.jp/link/ui/2008264798
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed