シスケイヒ酸アナログの植物生長阻害活性を転写産物レベルで評価する Transcriptomic evaluation of the enhanced plant growth-inhibitory activity caused by derivatization of <i>cis</i>-cinnamic acid
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To establish a rapid high-throughput evaluation system for the enhanced plant growth-inhibitory activity caused by modifications of <i>cis</i>-cinnamic acid's (<i>cis</i>-CA's) chemical structure, a DNA microarray assay was used to analyze the changes in early gene responses of <i>Arabidopsis thaliana</i> seedlings. After a 6-hr exposure to (<i>Z</i>)-3-(3-iodophenyl)acrylic acid, we observed an upregulation in three classes of early auxin-responsive genes, which was similar to the transcriptional response to indole-3-acetic acid (IAA), together with an upregulation of the genes related to environmental stress and toxin detoxification responses. Gene responses to 2-(3,4-dihydronaphthalen-1-yl)acetic acid were similar to those to IAA. In contrast, fewer genes were upregulated in response to its double-bond isomer, (<i>Z</i>)-2-[3,4-dihydronaphthalen-1(2<i>H</i>)-ylidene]acetic acid, than to <i>cis</i>-CA. DNA microarray data suggest that the structurally different <i>cis</i>-CA analogues trigger diverse gene responses.
- Journal of Pesticide Science
Journal of Pesticide Science 39(2), 85-90, 2014
Pesticide Science Society of Japan