トラフグ病魚から分離された非運動性Tenacibaculum maritimumの生物学的および血清学的性状  [in Japanese] Biological and Serological Characterization of a Non-gliding Strain of <i>Tenacibaculum maritimum</i> Isolated from a Diseased Puffer Fish <i>Takifugu rubripes</i>  [in Japanese]

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Abstract

滑走細菌症に罹患したトラフグから通常の樹根状コロニーに混じって淡黄色小型円形コロニーを形成するT. maritimumが分離された。その代表株NUF1129について生物学的および血清学的性状を調べたところ,本菌株は非運動性で,ヒラメに対して病原性が認められなかった。液体培養の際にフラスコ壁に付着しにくく,魚体表面への付着菌数も強毒株に比べて少なかった。ゲル内沈降反応において運動性株が共通に保有した菌体表在性抗原の1つが検出されず,全菌体タンパク質のSDS-ポリアクリルアミドゲル電気泳動で運動性株と異なるバンドパターンを示した。以上のことから,運動性や付着性に関連した菌体表在性物質がT. maritimumの病原性に関与することが示唆された。

<i>Tenacibaculum maritimum</i> is a Gram-negative, gliding marine bacterium that causes tenacibaculosis, an ulcerative disease of marine fish. The bacterium usually forms rhizoid colonies on agar media. We isolated <i>T. maritimum</i> that formed slightly yellowish round compact colonies together with the usual rhizoid colonies from a puffer fish <i>Takifugu rubripes</i> suffering from tenacibaculosis, and studied the biological and serological characteristics of a representative isolate of the compact colony phenotype, designated strain NUF1129. The strain was non-gliding and avirulent in Japanese flounder <i>Paralichthys olivaceus</i> in immersion challenge test and showed lower adhesion ability to glass wall in shaking broth culture and to the body surface of flounder. It lacked a cell-surface antigen commonly detected in gliding strains of the bacterium in gel immunodiffusion tests. SDS-PAGE analysis showed different polypeptide banding patterns between NUF1129 and gliding strains. Like gliding strains, NUF1129 exhibited both chondroitinase and gelatinase activities, which are potential virulence factors of the bacterium. These results suggest that some cell-surface components related to gliding and adhesion ability are implicated in the virulence of <i>T. maritimum</i>.

Journal

  • Fish Pathology

    Fish Pathology 49(3), 121-129, 2014

    The Japanese Society of Fish Pathology

Codes

  • NII Article ID (NAID)
    130004785808
  • NII NACSIS-CAT ID (NCID)
    AN00063165
  • Text Lang
    JPN
  • Article Type
    journal article
  • ISSN
    0388-788X
  • NDL Article ID
    025842458
  • NDL Call No.
    Z18-994
  • Data Source
    NDL  IR  J-STAGE  JASI 
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