Role of bottom-fermenting brewer's yeast KEX2 in high temperature resistance and poor proliferation at low temperatures

DOI Web Site 4 Citations 21 References
  • Yamagishi Hiromi
    Research Laboratories of Brewing Technology, Asahi Breweries, LTD.
  • Ohnuki Shinsuke
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
  • Nogami Satoru
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
  • Ogata Tomoo
    Research Laboratories of Brewing Technology, Asahi Breweries, LTD.
  • Ohya Yoshikazu
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo

Abstract

Variants of bottom-fermenting brewer’s yeast that grew at high temperatures and showed poor proliferation and fermentation at low temperatures were isolated. Similar variants of laboratory yeast were also isolated and found to be incapable of mating. The KEX2 gene was cloned by complementation. It was shown to be responsible for these traits, because a KEX2 disruptant of Saccharomyces cerevisiae (S. cerevisiae) laboratory yeast grew poorly at low temperatures and was resistant to high temperatures. In addition, a Saccharomyces bayanus (S. bayanus)-type KEX2 (Sb-KEX2) disruptant of bottom-fermenting brewer’s yeast grew poorly at low temperatures and was resistant to high temperatures. The KEX2 gene product plays an important role in proliferation of yeast at low temperatures, which is an important trait of bottom-fermenting brewer’s yeast. These findings advance our understanding of the proliferation of yeast at low temperatures, especially that of bottom-fermenting brewer’s yeast.

Journal

Citations (4)*help

See more

References(21)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top