Expression of the serum opacity factor gene and the variation in its upstream region in Streptococcus dysgalactiae isolates from fish
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- Nishiki Issei
- Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki
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- Minami Takayuki
- Miyazaki Prefectural Fisheries Experimental Station
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- Chen Shih-Chu
- Department of Veterinary Medicine, National Pingtung University of Science and Technology Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology
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- Itami Toshiaki
- Faculty of Agriculture, University of Miyazaki
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- Yoshida Terutoyo
- Faculty of Agriculture, University of Miyazaki
書誌事項
- タイトル別名
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- Expression of the serum opacity factor gene and the variation in its upstream region in <i>Streptococcus</i> <i>dysgalactiae</i> isolates from fish
抄録
Group C Streptococcus dysgalactiae (GCSD) is a pathogen of farmed fish. Almost all GCSD isolates from Asian countries, including Japan, Taiwan, Malaysia, and China, have a serum opacity factor (SOF-FD). Although the SOF-FD sequences in different GCSD isolates are identical, different opacification activities are observed. Three types of variations were observed in the upstream sequence of the sof-FD gene in GCSD isolates with different SOF-FD activities. Type 1 was characterized by insertion of an IS981-like element into the upstream region of the sof-FD gene. In Type 2, an IS981-like element was inserted into the upstream region in a direction opposite to that in Type 1. In Type 3, no IS element was inserted. Type 1 was predominant among Japanese isolates (129 of 133). Isolates from other Asian countries were generally Type 3 (13 of 16). Except for 1 strain, Type 1 strains exhibited opacification activities with optical densities (ODs)>0.6, while Type 2 and Type 3 strains have low opacification activities (ODs >0.2). Only Type 1 strains have putative −10 and −35 promoter regions upstream of the sof-FD gene, and the expression level of the sof-FD gene was higher in Type 1 strains than in Type 2 and Type 3 strains.
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 58 (6), 457-463, 2012
公益財団法人 応用微生物学・分子細胞生物学研究奨励会