A novel small regulatory RNA enhances cell motility in enterohemorrhagic <i>Escherichia coli</i>

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Author(s)

    • Sudo Naoki
    • Department of Life Science, College of Science, Rikkyo University
    • Hayashi Tetsuya
    • Division of Microbial Genomics, Department of Genomics and Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki
    • Kurokawa Ken
    • Earth-Life Science Institute, Tokyo Institute of Technology
    • Ohnishi Makoto
    • Department of Bacteriology, National Institute of Infectious Diseases
    • Muto Akira
    • Department of Biochemistry and Molecular Biology, Faculty of Agriculture and Life Science, Hirosaki University
    • Iyoda Sunao
    • Department of Bacteriology, National Institute of Infectious Diseases
    • Suh Mayumi
    • Department of Life Science, College of Science, Rikkyo University
    • Abe Hiroyuki
    • Department of Biomedical Informatics, Osaka University Graduate School of Medicine
    • Tobe Toru
    • Department of Biomedical Informatics, Osaka University Graduate School of Medicine
    • Ogura Yoshitoshi
    • Division of Microbial Genomics, Department of Genomics and Bioenvironmental Science, Frontier Science Research Center, University of Miyazaki

Abstract

Small regulatory RNAs (sRNAs) are conserved among a wide range of bacteria. They modulate the translational efficiency of target mRNAs through base-pairing with the help of RNA chaperone Hfq. The present study identified a novel sRNA, Esr41 (<u>e</u>nterohemorrhagic <i>Escherichia coli</i> O157 <u>s</u>mall <u>R</u>NA #<u>41</u>), from an intergenic region of an enterohemorrhagic <i>E. coli</i> (EHEC) O157:H7 Sakai-specific sequence that is not present in the nonpathogenic <i>E. coli</i> K-12. Esr41 was detected as an RNA molecule approximately 70 nucleotides long with a 3′ GC-rich palindrome sequence followed by a long poly(U), which is a characteristic of rho-independent terminators and is also a structural feature required for the action of Hfq. EHEC O157 harboring a multicopy plasmid carrying the <i>esr41</i> gene increased cell motility and the expression of <i>fliC</i>, a gene encoding a major flagellar component. These results indicate that Esr41 stimulates <i>fliC</i> expression in EHEC O157. Furthermore, the increase in cell motility induced by Esr41 was also observed in the <i>E. coli</i> K-12, suggesting that target genes controlled by Esr41 are present in both EHEC O157 and K-12.

Journal

  • The Journal of General and Applied Microbiology

    The Journal of General and Applied Microbiology 60(1), 44-50, 2014

    Applied Microbiology, Molecular and Cellular Biosciences Research Foundation

Codes

  • NII Article ID (NAID)
    130004802264
  • Text Lang
    ENG
  • ISSN
    0022-1260
  • Data Source
    J-STAGE 
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