Regulation of protein expression in relation with ovarian clear cell adenocarcinoma

• Arakawa Noriaki

  Yokohama City University, Graduate School of Nanobioscience

• Masuishi Yusuke

  Yokohama City University, Graduate School of Nanobioscience

• Hirano Hisashi

  Yokohama City University, Graduate School of Nanobioscience Yokohama City University, Advanced Medical Research Center

Bibliographic Information

Other Title

• 卵巣明細胞腺がん関連タンパク質の発現調節

Abstract

Proteomics is a useful aid to discover new diagnosis markers or drug-targets for various diseases. To identify proteins expressed specifically in ovarian clear cell adenocarcinoma (CCA), a malignant strain of ovarian cancer subtype, we compared the proteomic patterns of cell lines derived from CCA and non-CCA using Two-Dimensional Differential Gel Electrophoresis (2D-DIGE) analysis. We identified several proteins, including annexin IV, which were increased in several CCA cell lines. These proteins were verified to be upregulated in several CCA cell lines by Western blot and quantitative RT-PCR analyses. Promoter analysis of annexin IV gene revealed that the p53-binding motif was involved in CCA-specific expression. Mutations in the p53 gene were absent in several CCA-derived cell lines, and gene silencing of p53 by RNA interference decreased the ANX4 expression in the CCA cell lines. Thus, the annexin IV gene is regulated by p53 in the CCA cells. Elevations of several p53-induced proteins were also observed in CCA cells. Therefore, we concluded that p53 functional status is involved in forming the unique features of the proteome in CCA cell lines.<br>

Journal

• SEIBUTSU BUTSURI KAGAKU

  SEIBUTSU BUTSURI KAGAKU 55 (1), 5-8, 2011

  Japanese Electrophoresis Society

Keywords

• disease proteomics
• 2D-DIGE
• ovarian clear cell adenocarcinoma
• annexin IV
• p53

Details

• CRID: 1390282679179997824
• NII Article ID: 130004809437
• DOI: 10.2198/sbk.55.5
• ISSN: 13499785
• ISSN: 00319082
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed