Phos-tagを用いた活性リン脂質の質量分析  [in Japanese] Mass spectrometric analysis of phospholipids with phosphate monoester residue using Phos-tag  [in Japanese]

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Author(s)

    • 田中 保 Tanaka Tamotsu
    • 徳島大学大学院ヘルスバイオサイエンス研究部(薬学系) Institute of Health Biosciences, University of Tokushima Graduate School
    • 徳村 彰 Tokumura Akira
    • 徳島大学大学院ヘルスバイオサイエンス研究部(薬学系) Institute of Health Biosciences, University of Tokushima Graduate School

Abstract

Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are growth factor-like bioactive lipids having a phosphate monoester residue. Phos-tag can bind to them and be used both for purification and quantification. In a two-phase solvent system consisting of chloroform/methanol/water, addition of Phos-tag move LPA and S1P from a hydrophilic phase to a hydrophobic phase in the form of their Phos-tag complexes. Using this property, we developed a method for purification of LPA and S1P in biological materials by the phase separation technique. Advantages of use of Phos-tag for detection of LPA and S1P in MALDI-TOF MS are an increase in ionization efficiency and detection as a single-ion form. Homologues of LPA and S1P in natural samples can be quantified by MALDI-TOF MS by using internal standards.<br>

Journal

  • SEIBUTSU BUTSURI KAGAKU

    SEIBUTSU BUTSURI KAGAKU 56(Suppl_1), S37-S40, 2012

    Japanese Electrophoresis Society

Codes

  • NII Article ID (NAID)
    130004809454
  • NII NACSIS-CAT ID (NCID)
    AN00129729
  • Text Lang
    JPN
  • ISSN
    0031-9082
  • NDL Article ID
    023553759
  • NDL Call No.
    Z18-127
  • Data Source
    NDL  J-STAGE 
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