Phos-tagを用いた活性リン脂質の質量分析 Mass spectrometric analysis of phospholipids with phosphate monoester residue using Phos-tag

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著者

    • 田中 保 Tanaka Tamotsu
    • 徳島大学大学院ヘルスバイオサイエンス研究部(薬学系) Institute of Health Biosciences, University of Tokushima Graduate School
    • 徳村 彰 Tokumura Akira
    • 徳島大学大学院ヘルスバイオサイエンス研究部(薬学系) Institute of Health Biosciences, University of Tokushima Graduate School

抄録

Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are growth factor-like bioactive lipids having a phosphate monoester residue. Phos-tag can bind to them and be used both for purification and quantification. In a two-phase solvent system consisting of chloroform/methanol/water, addition of Phos-tag move LPA and S1P from a hydrophilic phase to a hydrophobic phase in the form of their Phos-tag complexes. Using this property, we developed a method for purification of LPA and S1P in biological materials by the phase separation technique. Advantages of use of Phos-tag for detection of LPA and S1P in MALDI-TOF MS are an increase in ionization efficiency and detection as a single-ion form. Homologues of LPA and S1P in natural samples can be quantified by MALDI-TOF MS by using internal standards.<br>

収録刊行物

  • 生物物理化学

    生物物理化学 56(Suppl_1), S37-S40, 2012

    日本電気泳動学会

各種コード

  • NII論文ID(NAID)
    130004809454
  • NII書誌ID(NCID)
    AN00129729
  • 本文言語コード
    JPN
  • ISSN
    0031-9082
  • NDL 記事登録ID
    023553759
  • NDL 請求記号
    Z18-127
  • データ提供元
    NDL  J-STAGE 
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