Pilocarpine-induced salivary fluid secretion in the perfused submandibular gland of the rat
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- Qi Bing
- Department of Physiology, Nihon University School of Dentistry at Matsudo
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- Narita Takanori
- Department of Physiology, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
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- Sugiya Hiroshi
- Department of Physiology, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
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- Murakami Masataka
- Department of Nano-structure of Physiology, National Institute for Physiological Sciences
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Abstract
Xerostomia is the symptom of dry mouth often seen in patients who receive head and neck radiation therapy or in patients who have Sjögren’s syndrome. The primary treatment to relieve xerostomia symptom is oral administration of pilocarpine, a parasympathomimetic agent with muscarinic action. Increase in salivary secretion induced by systemic administration of pilocarpine is considered to be mediated by actions on muscarinic cholinergic receptors in the central nervous system and salivary glands. In this study, we investigated the direct effect of pilocarpine on salivary fluid secretion in the isolated, perfused rat submandibular gland. Pilocarpine provoked salivary fluid secretion in a dose-dependent manner. The Na+-channel blocker tetrodotoxin had almost no effect on the pilocarpine-induced salivary fluid secretion, indicating that pilocarpine directly stimulates submandibular gland. Pilocarpine induced an increase in intracellular Ca2+ concentration in dispersed submandibular gland cells at 37°C , but not 25°C. The salivary fluid secretion induced by pilocarpine was consisted of a rapid and transient phase and a subsequent sustained phase, which profile was different from that evoked by carbachol, another typical muscarinic agonist. Pilocarpine also induced Lucifer yellow secretion via paracellular route. J. Med. Invest. 56 Suppl.: 281-283, December, 2009
Journal
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- The Journal of Medical Investigation
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The Journal of Medical Investigation 56 (Supplement), 281-283, 2009
The University of Tokushima Faculty of Medicine
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Details 詳細情報について
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- CRID
- 1390001204243003648
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- NII Article ID
- 130004822617
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- NII Book ID
- AA11166929
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- ISSN
- 13496867
- 13431420
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed