Effects of Slc26a6 deletion and CFTR inhibition on HCO3- secretion by mouse pancreatic duct

• Song Ying

  Second Clinical College of Norman Bethune Medical Division, Jilin University Department of Human Nutrition, Nagoya University Graduate School of Medicine

• Ishiguro Hiroshi

  Department of Human Nutrition, Nagoya University Graduate School of Medicine

• Yamamoto Akiko

  Department of Human Nutrition, Nagoya University Graduate School of Medicine

• Xiang Jin Chun

  Second Clinical College of Norman Bethune Medical Division, Jilin University

• Kondo Takaharu

  Department of Human Nutrition, Nagoya University Graduate School of Medicine

Abstract

Pancreatic duct epithelium secretes HCO₃^--rich fluid, which is dependent on cystic fibrosis transmembrane conductance regulator (CFTR). HCO₃^- transport across the apical membrane is thought to be mediated by both SLC26A6 Cl^--HCO₃^- exchange and CFTR HCO₃^- conductance. In this study we examined the relative contribution and interaction of SLC26A6 and CFTR in apical HCO₃^- transport. Interlobular pancreatic ducts were isolated from slc26a6 null mice. Intracellular pH (pH_i) was measured by BCECF microfluorometry. Duct cells were stimulated with forskolin and alkalinized by acetate pre-pulse in the presence of HCO₃^--CO₂. Apical HCO₃^- secretion was estimated from the recovery rate of pH_i from alkaline load. When the lumen was perfused with high-Cl^- solution, the rate of apical HCO₃^- secretion was increased by luminal application of CFTRinh-172 in ducts from wild-type mice but it was decreased in ducts from slc26a6 -/- mice. This suggests that slc26a6 and CFTR compensate/compete with each other for apical HCO₃^- secretion with high Cl^- in the lumen. With high HCO₃^- in the lumen, luminal CFTRinh-172 reduced the rate of apical HCO₃^- secretion in both wild-type and slc26a6 -/- ducts. This suggests that HCO₃^- conductance of CFTR mediates a significant portion of apical HCO₃^- secretion with high HCO₃^- in the lumen. J. Med. Invest. 56 Suppl.: 332-335, December, 2009

Journal

• The Journal of Medical Investigation

  The Journal of Medical Investigation 56 (Supplement), 332-335, 2009

  The University of Tokushima Faculty of Medicine

Keywords

• pancreatic duct cell
• HCO<SUB>3</SUB><SUP>-</SUP> transport
• CFTR
• slc26A6

Details

• CRID: 1390282679219743488
• NII Article ID: 130004822632
• NII Book ID: AA11166929
• DOI: 10.2152/jmi.56.332
• ISSN: 13496867
• ISSN: 13431420
• Web Site: https://repo.lib.tokushima-u.ac.jp/111456
• Web Site: http://www.jstage.jst.go.jp/article/jmi/56/Supplement/56_Supplement_332/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010325512
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed