Mineral to matrix ratio determines biomaterial and biomechanical properties of rat femur -application of Fourier transform infrared spectroscopy-

• Takata Shinjiro

  Division of Rehabilitation, Tokushima University Hospital

• Yonezu Hiroshi

  Department of Orthopedic Surgery, Mitoyo General Hospital

• Shibata Akira

  Faculty of Pharmaceutical Science, Yasuda Women’s University

• Enishi Tetsuya

  Division of Rehabilitation, Tokushima University Hospital

• Sato Nori

  Division of Rehabilitation, Tokushima University Hospital

• Takahashi Mitsuhiko

  Department of Orthopedics, Institute of Health Biosciences, the University of Tokushima Graduate School

• Nakao Shigetaka

  Division of Rehabilitation, Tokushima University Hospital

• Komatsu Koji

  Division of Rehabilitation, Tokushima University Hospital

• Yasui Natsuo

  Division of Rehabilitation, Tokushima University Hospital Department of Orthopedics, Institute of Health Biosciences, the University of Tokushima Graduate School

Abstract

We studied the changes of biomaterial and biomechanical properties of the rat femur during development. Thirty male Wistar rats were allocated to 6 groups: aged 6 weeks (n=5), 9 weeks (n=5), 12 weeks (n=5), 15 weeks (n=5), 24 weeks (n=5), and 36 weeks (n=5). The mineral to matrix ratio (M/M ratio) of rat femur by Fourier transform infrared spectroscopy was 0.97±0.10 at the age of 6 weeks, and reached the maximum of 1.52±0.17 at the age of 36 weeks. Total bone mineral density (BMD) by peripheral quantitative computed tomography of the femoral shaft aged 6 weeks was 479.1±58.7 mg/cm³, and reached the maximum of 1022.2±42.3 mg/cm³ at the age of 36 weeks. The ultimate load to failure of the femur of the rat aged 6 weeks by the three-point bending test was 29.6±6.1 N. At the age of 36 weeks, the ultimate load to failure of the rat femur increased to the maximum of 283.5±14.7 N. The results showed that the M/M ratio increased with development as total BMD and bone strength increased. The results suggest that the M/M ratio is one of the determinants of the biomaterial and biomechanical properties of bone. J. Med. Invest. 58: 197-202, August, 2011

Journal

• The Journal of Medical Investigation

  The Journal of Medical Investigation 58 (3,4), 197-202, 2011

  The University of Tokushima Faculty of Medicine

Keywords

• Fourier transform infrared spectroscopy
• bone quality
• peripheral quantitative computed tomography
• three-point bending test

Details

• CRID: 1390001204245005696
• NII Article ID: 130004822657
• NII Book ID: AA11166929
• DOI: 10.2152/jmi.58.197
• ISSN: 13496867
• ISSN: 13431420
• Web Site: http://repo.lib.tokushima-u.ac.jp/82891
• Web Site: http://www.jstage.jst.go.jp/article/jmi/58/3,4/58_3,4_197/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012323184
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed