Effect of Kampo medicine “Dai-kenchu-to” on microbiome in the intestine of the rats with fast stress

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Author(s)

    • Yoshikawa Kozo
    • Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Higashijima Jun
    • Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Kashihara Hideya
    • Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Takasu Chie
    • Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Matsumoto Noriko
    • Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Shimada Mitsuo
    • Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Kurita Nobuhiro
    • Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Sato Hirohiko
    • Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Utsunomiya Tohru
    • Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Iwata Takashi
    • Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
    • Miyatani Tomohiko
    • Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School

Abstract

[Purpose] Diversity of gut microbiome has been recently reported to be lost in inflammatory bowel disease. We have previously reported that the Dai-kenchu-to (DKT) prevented the bacterial translocation through suppression of cytokine and apoptosis in rat’s fast stress model. The aim of this study was to evaluate the effect of DKT on maintenance of microbial diversity in rat’s intestine with inflammation. [Method] Wister rats were received the fast stress for 5 days. In DKT group, rats were administered with DKT (300 mg/kg/day) during the fast stress (DKT-group). The gut microbiomes were analyzed at before- and after- fast stress, and the effect of DKT for on microbial diversities of the gut were evaluated by the PCR-clone library method targeting the 16 S ribosomal RNA gene. [Result] In Control-group, Erysipelotrichaceae increased to 86% in after fast stress, OTU of before-fast stress was 111 and after fast stress was only 9 (changing rate: 58%). The diversity of microbiome was severely decreased. On the other hand, in DKT-group, diversity of microbiome was kept after fast stress (<I>Lachnospiraceae: Ruminococcaceae: Coriobacteriales</I> 54%, 22%, 5%), Operational taxonomic units of before fast stress was 52 and after fast stress was 55 (changing rate: 6%). Family <I>Lachnospiraceae</I> which includes butyrate-producing Clostridia (Clostridium IV and XIVa). [Conclusion] DKT prevented the reduction of diversity of microbiome in rat’s fast stress model. Our data suggested the new anti-inflammatory mechanism of DKT through gut microbiome. J. Med. Invest. 60: 221-227, August, 2013

Journal

  • The Journal of Medical Investigation

    The Journal of Medical Investigation 60(3.4), 221-227, 2013

    The University of Tokushima Faculty of Medicine

Codes

  • NII Article ID (NAID)
    130004822686
  • NII NACSIS-CAT ID (NCID)
    AA11166929
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1343-1420
  • Data Source
    IR  J-STAGE 
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