Effect of Kampo medicine “Dai-kenchu-to” on microbiome in the intestine of the rats with fast stress

DOI IR Web Site PubMed 3 Citations 24 References
  • Yoshikawa Kozo
    Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Shimada Mitsuo
    Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Kuwahara Tomomi
    Department of Microbiology, Faculty of Medicine, Kagawa University
  • Hirakawa Hideki
    Kazusa DNA Research Institute
  • Kurita Nobuhiro
    Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Sato Hirohiko
    Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Utsunomiya Tohru
    Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Iwata Takashi
    Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Miyatani Tomohiko
    Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Higashijima Jun
    Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Kashihara Hideya
    Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Takasu Chie
    Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Matsumoto Noriko
    Department of Surgery, Institute of Health Biosciences, the University of Tokushima Graduate School
  • Nakayama-Imaohji Haruyuki
    Department of Microbiology, Faculty of Medicine, Kagawa University

Search this article

Abstract

[Purpose] Diversity of gut microbiome has been recently reported to be lost in inflammatory bowel disease. We have previously reported that the Dai-kenchu-to (DKT) prevented the bacterial translocation through suppression of cytokine and apoptosis in rat’s fast stress model. The aim of this study was to evaluate the effect of DKT on maintenance of microbial diversity in rat’s intestine with inflammation. [Method] Wister rats were received the fast stress for 5 days. In DKT group, rats were administered with DKT (300 mg/kg/day) during the fast stress (DKT-group). The gut microbiomes were analyzed at before- and after- fast stress, and the effect of DKT for on microbial diversities of the gut were evaluated by the PCR-clone library method targeting the 16 S ribosomal RNA gene. [Result] In Control-group, Erysipelotrichaceae increased to 86% in after fast stress, OTU of before-fast stress was 111 and after fast stress was only 9 (changing rate: 58%). The diversity of microbiome was severely decreased. On the other hand, in DKT-group, diversity of microbiome was kept after fast stress (Lachnospiraceae: Ruminococcaceae: Coriobacteriales 54%, 22%, 5%), Operational taxonomic units of before fast stress was 52 and after fast stress was 55 (changing rate: 6%). Family Lachnospiraceae which includes butyrate-producing Clostridia (Clostridium IV and XIVa). [Conclusion] DKT prevented the reduction of diversity of microbiome in rat’s fast stress model. Our data suggested the new anti-inflammatory mechanism of DKT through gut microbiome. J. Med. Invest. 60: 221-227, August, 2013

Journal

Citations (3)*help

See more

References(24)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top