Preventive effects of metallothionein against DNA and lipid metabolic damages in dyslipidemic mice under repeated mild stress

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Author(s)

    • Higashimoto Minoru
    • Faculty of Pharmaceutical Sciences, Tokushima Bunri University|AWA Laboratory of Health-Food Drinks
    • Ogawa Naoko
    • Faculty of Human Life Sciences, Tokushima Bunri University
    • Takiguchi Masufumi
    • Faculty of Pharmaceutical Sciences, Tokushima Bunri University|Faculty of Pharmaceutical Sciences, Hiroshima International University
    • Suzuki Shinya
    • Faculty of Pharmaceutical Sciences, Tokushima Bunri University
    • Sato Masao
    • Faculty of Pharmaceutical Sciences, Tokushima Bunri University

Abstract

The effects of repeated mild stress on DNA and lipid metabolic damages in multiple organs of dyslipidemic mice, and the preventive role of metallothionein (MT) were investigated. Female adult wild-type and MT-null mice fed high-fat diet (HFD) or standard diet (STD) were repeatedly subjected to fasting or restraint for three weeks. The liver, pancreas, spleen, bone marrow and serum samples were taken for evaluating DNA damage, MT, glutathione (GSH), corticosterone, carnitine and adiponectin. Body weights of restraint groups were reduced with the intensity of stress increased, even if the energy intakes were higher than those of STD group. Hepatic GSH levels were reduced in HFD control group and were further reduced in stress groups, especially in restraint groups, while the hepatic MT and serum corticosterone levels were increased in concert with the intensity of stress. Cellular DNA damages were generally increased by the restraint stress, especially in MT-null mice. Hepatic carnitine levels of MT-null mice were markedly lower than those of wild-type mice. The data suggest that MT plays a preventive role by acting as an antioxidant in corporation with GSH decreased by repeated stress and that MT may be an essential factor for inducing carnitine under the stress. J. Med. Invest. 60: 240-248, August, 2013

Journal

  • The Journal of Medical Investigation

    The Journal of Medical Investigation 60(3.4), 240-248, 2013

    The University of Tokushima Faculty of Medicine

Codes

  • NII Article ID (NAID)
    130004822689
  • NII NACSIS-CAT ID (NCID)
    AA11166929
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1343-1420
  • Data Source
    IR  J-STAGE 
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