Growth properties of macaque-tropic HIV-1 clones carrying vpr/vpx genes derived from simian immunodeficiency viruses in place of their vpr regions

• Doi Naoya

  Department of Microbiology, Institute of Health Biosciences, the University of Tokushima Graduate School Japanese Foundation for AIDS Prevention

• Adachi Akio

  Department of Microbiology, Institute of Health Biosciences, the University of Tokushima Graduate School

• Nomaguchi Masako

  Department of Microbiology, Institute of Health Biosciences, the University of Tokushima Graduate School

書誌事項

タイトル別名

• Growth properties of macaque-tropic HIV-1 clones carrying <i>vpr/vpx </i>genes derived from simian immunodeficiency viruses in place of their <i>vpr </i>regions

抄録

We have previously generated a macaque-tropic human immunodeficiency virus type 1 (HIV-1mt) clone designated MN4/LSDQgtu by genetic manipulation from a parental virus that replicates poorly in rhesus macaque cells. In rhesus cell line M1.3S and peripheral blood mononuclear cells (PBMCs), MN4/LSDQgtu grows comparably to a standard simian immunodeficiency virus clone derived from the rhesus macaque (SIVmac239) that can induce the acquired immunodeficiency syndrome (AIDS) in the animals. In this study, we further modified the Vpr-coding region of MN4/LSDQgtu genome by introducing vpr gene of an SIV clone from the greater spot-nosed monkey (SIVgsn166) or vpx gene of SIVmac239 to generate four new clones for determining functional importance of the central genomic area. Furthermore, two clones with an additional Gag-p6 mutation were made to ensure the virion-packaging of Vpx. In addition, accessory gene mutant clones of MN4/LSDQgtu with a frame-shift mutation, including a vpr mutant, were constructed and their growth properties were examined. Infection experiments showed that newly constructed viruses all grew poorly to various degrees in M1.3S cells, relative to MN4/LSDQgtu. Together with the previous data, our results here show that vpr/vpx gene in the appropriate context of HIV-1 genome is critical for viral growth ability. J. Med. Invest. 61: 374-379, August, 2014

収録刊行物

• The Journal of Medical Investigation

  The Journal of Medical Investigation 61 (3.4), 374-379, 2014

  国立大学法人　徳島大学医学部

キーワード

• HIV-1mt
• SIV
• vpx
• vpr

詳細情報

• CRID: 1390282679219928576
• NII論文ID: 130004822744
• NII書誌ID: AA11166929
• DOI: 10.2152/jmi.61.374
• ISSN: 13496867
• ISSN: 13431420
• PubMed: 25264057
• Web Site: http://repo.lib.tokushima-u.ac.jp/109587
• Web Site: https://www.jstage.jst.go.jp/article/jmi/61/3.4/61_374/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015381627
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可