Mix-mode TiO-C18 Monolith Spin Column Extraction and GC-MS for the Simultaneous Assay of Organophosphorus Compounds and Glufosinate, and Glyphosate in Human Serum and Urine

• SAITO Takeshi

  Department of Emergency and Critical Care Medicine, Tokai University School of Medicine

• AOKI Hiromichi

  Department of Emergency and Critical Care Medicine, Tokai University School of Medicine

• NAMERA Akira

  Department of Forensic Medicine, Graduate School of Biomedical Sciences, Hiroshima University

• OIKAWA Hiroshi

  GL Sciences Inc.

• MIYAZAKI Shota

  GL Sciences Inc.

• NAKAMOTO Akihiro

  Forensic Science Laboratory, Hiroshima Prefectural Police Headquarters

• INOKUCHI Sadaki

  Department of Emergency and Critical Care Medicine, Tokai University School of Medicine

Abstract

A rapid, specific, and sensitive method for the simultaneous quantitation of organophosphates (fenitrothion (MEP), malathion, and phenthoate (PAP)), glufosinate (GLUF), and glyphosate (GLYP) in human serum and urine by gas chromatography–mass spectrometry (GC-MS) has been validated. All of the targeted compounds together with the internal standard were extracted from the serum and urine using a mix-mode TiO-C₁₈ monolithic spin column. The recovery of organophosphates from serum and urine ranged from 12.7 to 49.5%. The recovery of GLUF and GLYP from serum and urine ranged from 1.9 to 7.9%. The intra- and inter-accuracy and precision (expressed as relative standard deviation, %RSD) were within 96.7 – 107.7% and 4.0 – 13.8%, respectively. The detection and quantitation limits for serum and urine were 0.1 and 0.1 μg/ml, respectively, for organophosphates, 0.1 and 0.5 μg/ml, respectively for GLUF and GLYP. The method had linear calibration curves ranging from 0.1 to 25.0 μg/ml for organophosphates and 0.5 – 100.0 μg/ml for GLUF, and GLYP. The validated method was successfully applied to a clinical GLYP poisoning case.

Journal

• Analytical Sciences

  Analytical Sciences 27 (10), 999-, 2011

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390282679238074880
• NII Article ID: 130004827065
• NII Article ID: 10029881081
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.27.999
• COI: 1:STN:280:DC%2BC3MbgtVWqtA%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 11264683
• PubMed: 21985924
• Web Site: http://id.ndl.go.jp/bib/11264683
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11264683
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.27.999.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.27.999/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/27/10/27_10_999/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012169830
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed