Determination of Cattle Foot-and-Mouth Disease Virus by Micro-ELISA Method
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- DONG Yiyang
- Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology
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- XU Yan
- Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century, Osaka Prefecture University Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)
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- LIU Zaixin
- Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory
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- FU Yuanfang
- Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory
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- OHASHI Toshinori
- Institute of Microchemical Technology (IMT) Co., Ltd.
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- MAWATARI Kazuma
- Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) Department of Applied Chemistry, School of Engineering, The University of Tokyo
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- KITAMORI Takehiko
- Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) Department of Applied Chemistry, School of Engineering, The University of Tokyo
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抄録
The development of foot-and-mouth disease virus (FMDV) detection methods is crucial for animal food security, tackling regional FMDV epidemic, and global FMDV prognostic control. For these purposes, a fast and sensitive analysis method is required. In this study, we developed a microchip-based ELISA (enzyme-linked immunosorbent assay), micro-ELISA, to realize FMDV detection. Nickel(II) chelating chemistry was utilized to immobilize recombinant protein (antigen) on polystyrene micro-beads in order to determine FMDV antibodies in cattle serum samples. In addition, reaction protocol and conditions were investigated. As a result, the FMDV detection was successfully demonstrated with only a 10-μL sample volume in 25-minute assay time. Analytical sensitivity was evaluated by a maximum nominal positiveness percentage value (NPPV) of 303 and a dilution factor of 32×. The method’s inter-run and intra-run CV (coefficients of variance) values were 15.5 and 17.1%, respectively, which were fully compatible with the OIE (World Organization for Animal Health) principle of validation of diagnosis assays for infectious diseases. The developed method should become a powerful tool for determining other animal contagious diseases and/or zoonosis.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 30 (3), 359-363, 2014
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204260589696
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- NII論文ID
- 130004827418
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC2crhslOhtg%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 025345435
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- PubMed
- 24614730
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- Web Site
- http://id.ndl.go.jp/bib/025345435
- https://ndlsearch.ndl.go.jp/books/R000000004-I025345435
- https://link.springer.com/content/pdf/10.2116/analsci.30.359.pdf
- https://link.springer.com/article/10.2116/analsci.30.359/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/30/3/30_359/_pdf
- https://search.jamas.or.jp/link/ui/2014236663
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 使用不可