Simple and Rapid Separation of Soyasaponin Bb from a Soy Extract

• KATANO Hajime

  Department of Bioscience, Fukui Prefectural University

• OKAMOTO Nobuhiro

  Department of Bioscience, Fukui Prefectural University

• TAKAKUWA Masahiro

  Department of Bioscience, Fukui Prefectural University

• TAIRA Shu

  Department of Bioscience, Fukui Prefectural University

• KAMBE Taiho

  Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University

• TAKAHASHI Masakazu

  Department of Bioscience, Fukui Prefectural University

抄録

A simple method to separate soyasaponin Bb from a soy extract is presented. This method is based on the difference in the solubility of soyasaponin Bb and Ba and other components into 3:7 and 1:1 (v/v) acetone–water mixed solvents. The crude soyasaponin consisting of soyasaponins Aa, Ab, Ba, and Bb at the 10 wt% level and other components was examined as the soy extract. A 10 mg quantity of the crude soyasaponin was mixed with 1 mL of the 3:7 acetone–water containing 0.1 mol/L HCl, and the supernatant was removed to obtain a precipitate, which was found to contain mainly soyasaponins Bb and Ba. The precipitate was mixed with 0.4 mL of the 1:1 acetone–water containing 0.1 mol/L HCl; the supernatant was transferred, and was mixed with 0.6 mL of water to obtain a precipitate, which was found to contain mainly soyasaponin Bb. The yield was ca. 30%, which may be much higher than that by the conventional preparative chromatographic approach. The separation method is rapid and easy to carry out, and is useful for the preparation of a soyasaponin Bb sample.

収録刊行物

• Analytical Sciences

  Analytical Sciences 31 (2), 85-89, 2015

  社団法人 日本分析化学会

キーワード

• Separation
• purification
• soyasaponin Bb
• soy extract
• organic–water mixed solvent

詳細情報

• CRID: 1390282679238211328
• NII論文ID: 130004827460
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.31.85
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 026098233
• PubMed: 25746805
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026098233
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.31.85.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.31.85/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/31/2/31_85/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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