Label-free Detection of Antigen Protein Using a Metal Mesh Device Surface-modified by an Antibody

• SETO Hirokazu

  Graduate School of Engineering, Kyushu University

• KAMBA Seiji

  Murata Manufacturing Co.

• KONDO Takashi

  Murata Manufacturing Co.

• OGAWA Yuichi

  Graduate School of Agriculture, Kyoto University

• HOSHINO Yu

  Graduate School of Engineering, Kyushu University

• MIURA Yoshiko

  Graduate School of Engineering, Kyushu University

抄録

A biosensor for protein detection was developed using antibody-immobilized metal mesh devices. Antihemoglobin antibodies were covalently immobilized on a metal mesh device. Extraordinary transmission with a dipped structure was observed for a metal mesh device immobilized with antihemoglobin antibodies as well as for the original metal mesh device. Hemoglobin in the mixture solution containing albumin at a hundred-fold concentration was detectable using antihemoglobin-immobilized MMDs. The detectability using the antihemoglobin-immobilized metal mesh device was similar to that of a commercially-available kit for the qualitative determination of hemoglobin.

収録刊行物

• Analytical Sciences

  Analytical Sciences 31 (3), 173-176, 2015

  社団法人 日本分析化学会

キーワード

• Metal mesh device
• antigen-antibody
• hemoglobin
• label-free biosensor

詳細情報

• CRID: 1390001204260263552
• NII論文ID: 130004827468
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.31.173
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 026247302
• PubMed: 25765271
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026247302
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.31.173.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.31.173/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/31/3/31_173/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015280986
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可