新規外来フジツボ <i>Perforatus perforatus</i> の日本への侵入確認およびリアルタイムPCR法を用いた検出方法について [in Japanese] The first find of the invasive alien barnacle <i>Perforatus perforatus</i> in Japan and the development of a detection method using real-time PCR [in Japanese]
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The acorn barnacle <i>Perforatus perforatus</i> is a common European species that is distributed from southwest England to the Mediterranean and the northwest coast of Africa. Here, we present the first record of invasion of <i>P. perforatus</i> in Japan. This species was found in September, 2012, at a high density on a buoy in Toga Bay in Akita Prefecture, northwest Honshu. It was identified based on the morphological characteristics of the shell and opercular valves, and by molecular analysis using a 300 bp nucleotide sequence of the mitochondrial 12S rRNA. Additionally, a detection and quantification method for <i>P. perforatus</i> using species-specific primer sets for quantitative real-time polymerase chain reaction was developed. Using this method, it was possible to identify and quantify the planktonic nauplius larvae of this alien species.
- Sessile Organisms
Sessile Organisms 32(1), 1-6, 2015
THE SESSILE ORGANISMS SOCIETY OF JAPAN