Application of Decellularized Liver for Oxygen Supplying Matrices

DOI 18 References
  • Funamoto Seiichi
    Department of material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University JST-CREST
  • Ago Yusuke
    Department of material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
  • Hashimoto Yoshihide
    Department of material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University JST-CREST
  • Negishi Jun
    Department of material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
  • Nam Kwangwoo
    Department of material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University JST-CREST
  • Kimura Tsuyoshi
    Department of material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University JST-CREST
  • Higami Tetsuya
    Department of cardiovascular surgery, Sapporo medical university of school of medicine
  • Kishida Akio
    Department of material-based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University JST-CREST

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Other Title
  • 酸素供給マトリクスとしての脱細胞化肝臓の応用

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Abstract

Blood supplying is one of the key factors for realizing the large-scale tissue and organ regeneration. One strategy is the use of acellular tissues which have capillary network by nature. In this study, we studied the condition of decellularization and capillary structure maintenance for applying the decellularized liver as the capillary network matrix for nutrient and oxygen supply. Rat liver was decellularized by using several kinds of detergents and high-hydrostatic pressure treatments. The decellularization of the liver was confirmed by hematoxylin-eosin staining. The perfusion of dye and the X-ray μCT scanning were used to confirm the capillary network structure of the decellularized liver. The performance of the decellularized livers as the perfusion base material to supply oxygen was evaluated. The best result was given by HHP treated liver. Oxygen supply through the tunica serosa hepatis of decellularized liver to outside was also capable. These results suggest that the decellularized liver could be applied as capillary network matrix.

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