Misfolded Glycoproteins as Probes for Analysis of Folding Sensor Enzyme UDP-Glucose

• Izumi Masayuki

  Japan Science and Technology Agency (JST), ERATO, Ito Glycotrilogy Project Department of Chemistry, Graduate School of Science, Osaka University

• Kiuchi Tatsuto

  Department of Chemistry, Graduate School of Science, Osaka University

• Ito Yukishige

  RIKEN Advanced Science Institute Japan Science and Technology Agency (JST), ERATO, Ito Glycotrilogy Project

• Kajihara Yasuhiro

  Japan Science and Technology Agency (JST), ERATO, Ito Glycotrilogy Project Department of Chemistry, Graduate School of Science, Osaka University

Bibliographic Information

Other Title

• フォールディングセンサー酵素UDP-グルコース
• Glycoprotein Glucosyltransferase
• 糖タンパク質グルコース転移酵素の機能解明のためのプローブとなる変性糖タンパク質

Abstract

Glycoprotein quality control system exists in the endoplasmic reticulum and discriminates and excludes misfolded glycoproteins. The key component of this system is UDP-glucose : glycoprotein glucosyltransferase (UGGT), which serves as a folding sensor as it can only monoglucosylate misfolded glycoproteins. Monoglucosylation serves as a tag for refolding assisted by lectin chaperones calnexin/calreticulin. To elucidate the recognition mechanism of misfolding by UGGT, various sophisticated misfolded glycoprotein models have been reported. Variety of model glycoproteins can be prepared by biological approaches although those are usually heterogeneous in both glycan and protein structures. Recently, we introduced a chemical approach for the synthesis of homogeneous misfolded glycoprotein. Chemical method can provide small glycoproteins but with homogeneous glycan and with intentionally manipulated protein 3D-structure. In this review, both chemical and biological approaches for the preparation of misfolded glycoprotein probes are discussed, which will give us an ability to gain further insights into the glycoprotein quality control system.

Journal

• Trends in Glycoscience and Glycotechnology

  Trends in Glycoscience and Glycotechnology 25 (141), 1-12, 2013

  FCCA(Forum: Carbohydrates Coming of Age)

Keywords

• glycoprotein quality control
• UGGT
• misfolded glycoprotein
• chemical synthesis

Details

• CRID: 1390282679345940608
• NII Article ID: 130004841363
• NII Book ID: AA10995236
• DOI: 10.4052/tigg.25.1
• ISSN: 18832113
• ISSN: 09157352
• NDL BIB ID: 024812653
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024812653
• Web Site: https://search.jamas.or.jp/link/ui/2014014500
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed