Investigation of the Biotinylation Method for Detecting Thyroid Carcinoma-specific IgM Antibodies and the Detectability of Carcinoma Cells

DOI
  • Fujioka Kouki
    Department of Molecular Cell Biology, Institute of DNA Medicine, The Jikei University School of Medicine
  • Oikawa Tomoe
    Department of Molecular Cell Biology, Institute of DNA Medicine, The Jikei University School of Medicine
  • Takeyama Hiroshi
    Department of Surgery, The Jikei University School of Medicine
  • Usui Ritsuko
    Department of Molecular Cell Biology, Institute of DNA Medicine, The Jikei University School of Medicine
  • Nomura Mayumi
    Department of Molecular Cell Biology, Institute of DNA Medicine, The Jikei University School of Medicine
  • Tomaru Keiko
    Department of Molecular Cell Biology, Institute of DNA Medicine, The Jikei University School of Medicine
  • Ikeda Keiichi
    Department of Molecular Cell Biology, Institute of DNA Medicine, The Jikei University School of Medicine
  • Manome Yoshinobu
    Department of Molecular Cell Biology, Institute of DNA Medicine, The Jikei University School of Medicine

この論文をさがす

抄録

Fine needle aspiration cytology and histological analyses provide remarkable precision and are used to obtain a definitive diagnosis of thyroid carcinoma. In order to improve the quality of life of patients, developing an early detection method, in addition to Papanicolaou staining, for diagnosing thyroid carcinomas is crucial. We herein demonstrate that optical spacer biotinylation of JT IgM antibodies (>900 kDa, pentamer), which detect sialylated fibronectin in thyroid cancers, and biotinylated antibodies can be used for fluorescent and enzymatic staining. We investigated the detectability of antigens in the SW1736 cell line and exfoliative cells obtained from a papillary carcinoma patient using two staining methods, phycoerythrin (PE) fluorescent and diaminobenzidine (DAB) enzymatic staining. The fluorescent staining method provided higher visibility of the antigens than conventional DAB enzymatic staining. Although Papanicolaou staining is required to make a definitive diagnosis of thyroid carcinoma, the fluorescent staining method is helpful for detecting thyroid carcinoma antigens rapidly and at a higher sensitivity. Moreover, the use of the biotinylation method of detecting high-molecular IgM will shed light on the utilization of IgM antibodies.

収録刊行物

  • bioimages

    bioimages 21 (0), 1-5, 2013

    日本バイオイメージング学会

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ