Preparation of Silica-Coated Quantum Dot Nanoparticle Colloid Solutions and Their Application in <i>in-vivo</i> Fluorescence Imaging

• Kobayashi Yoshio

  Department of Biomolecular Functional Engineering, College of Engineering, Ibaraki University

• Matsudo Hiromu

  Department of Biomolecular Functional Engineering, College of Engineering, Ibaraki University

• Kubota Yohsuke

  Division of Surgical Oncology, Graduate School of Medicine, Tohoku University

• Nakagawa Tomohiko

  Division of Surgical Oncology, Graduate School of Medicine, Tohoku University

• Gonda Kohsuke

  Division of Surgical Oncology, Graduate School of Medicine, Tohoku University

• Ohuchi Noriaki

  Division of Surgical Oncology, Graduate School of Medicine, Tohoku University

Bibliographic Information

Other Title

• Preparation of Silica-Coated Quantum Dot Nanoparticle Colloid Solutions and Their Application in in-vivo Fluorescence Imaging

Abstract

This paper describes three findings. The first is a method for producing colloidal solutions of quantum dot (QD) nanoparticles with silica shells (QD/SiO₂). QD nanoparticles averaging 10.3±2.1 nm in size were coated with silica via a sol–gel reaction with tetraethyl orthosilicate using NaOH as a catalyst. The QD/SiO₂ particle size could be varied by varying the QD concentration. The average particle sizes were 19.1±3.0 (S-QD/SiO₂) and 47.0±6.1 nm (L-QD/SiO₂) for QD concentrations of 6.4×10⁻⁹ M (4.6×10¹¹ particles/L) and 6.4×10⁻¹⁰ M (4.6×10¹⁰ particles/L), respectively. The second finding is a method to modify the particle surface with poly(ethylene glycol), which is called PEGylation (QD/SiO₂/PEG). S-QD/SiO₂ and L-QD/SiO₂ were PEGylated using methoxy polyethylene glycol silane (S-QD/SiO₂/PEG and L-QD/SiO₂/PEG, respectively). The third finding is an in-vivo fluorescence imaging technique using the QD/SiO₂/PEG particle colloid solutions. Both QD/SiO₂/PEG particle colloid solutions fluoresced with intensities comparable with that of the QD colloid solution. Mouse tissues could be imaged by injecting the QD/SiO₂/PEG colloid solution into them and measuring the emitted fluorescence intensity. The L-QD/SiO₂/PEG particles did not form aggregates in blood, which allowed the particles to reach the tissues more efficiently than the S-QD/SiO₂/PEG particles.

Journal

• JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

  JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 48 (2), 112-117, 2015

  The Society of Chemical Engineers, Japan

Keywords

• Quantum Dot
• Silica-Coating
• Fluorescence Imaging
• Core–Shell
• Nanoparticle

Details

• CRID: 1390001204569861248
• NII Article ID: 130004862051
• NII Book ID: AA00709658
• DOI: 10.1252/jcej.14we218
• ISSN: 18811299
• ISSN: 00219592
• NDL BIB ID: 026286569
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026286569
• Web Site: https://www.jstage.jst.go.jp/article/jcej/48/2/48_14we218/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed