A Rapid and Quantitative Detection of Cellular Protein Kinase Activity Based on MALDI-TOF-MS

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  • Yuki Otsubo
    Department of Applied Chemistry, Faculty of Engineering, Kyushu University
  • Hiromu Ikeda
    Department of Applied Chemistry, Faculty of Engineering, Kyushu University
  • Junpei Kamimoto
    Department of Applied Chemistry, Faculty of Engineering, Kyushu University
  • Takuro Niidome
    Department of Applied Chemistry, Faculty of Engineering, Kyushu University
  • Takeshi Mori
    Department of Applied Chemistry, Faculty of Engineering, Kyushu University
  • Yoshiki Katayama
    Department of Applied Chemistry, Faculty of Engineering, Kyushu University

抄録

<jats:title>Abstract</jats:title> <jats:p>A rapid and quantitative measurement of protein kinase activity based on MALDI-TOF-MS was developed. Here, biotinylated substrate peptides with stable isotope labeling were used to enable the rapid and specific enrichment of the peptides. Protein kinase activities in two samples can be directly compared utlizing the mass-unit difference of the subsrate peptides with different isotope labels. We successfully evaluated the effect of an inhibitory drug on the cellular protein kinase acitivity.</jats:p>

収録刊行物

  • Chemistry Letters

    Chemistry Letters 43 (5), 658-660, 2014-01-25

    Oxford University Press (OUP)

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