Formation of Thin Molecularly Imprinted Hydrogel Layers with Lectin Recognition Sites on SPR Sensor Chips by Atom Transfer Radical Polymerization

• Yoshiaki Kuriu

  Faculty of Chemistry, Materials and Bioengineering, Kansai University

• Akifumi Kawamura

  Faculty of Chemistry, Materials and Bioengineering, Kansai University

• Tadashi Uragami

  Faculty of Chemistry, Materials and Bioengineering, Kansai University

• Takashi Miyata

  Faculty of Chemistry, Materials and Bioengineering, Kansai University

抄録

We prepared lectin-imprinted hydrogel layers on surface plasmon resonance (SPR) sensor chips by atom transfer radical polymerization (ATRP). In a buffer solution with a target protein, the SPR signal and binding constant of the lectin-imprinted hydrogel layer chip were much larger than those of the nonimprinted hydrogel layer chip prepared without using a template lectin. By molecular imprinting with ATRP, recognition sites for the target lectin were formed within thin hydrogel layers on the SPR sensor chips. The lectin-imprinted hydrogel layer chips with recognition sites for target protein are candidates for highly sensitive sensor devices for an SPR sensing system.

収録刊行物

• Chemistry Letters

  Chemistry Letters 43 (6), 825-827, 2014-03-01

  Oxford University Press (OUP)

詳細情報

• CRID: 1360565169058931968
• NII論文ID: 130004868186
• DOI: 10.1246/cl.140103
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/43/6/825/55641909/cl.140103.pdf
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