Alteration of DNAzyme Activity by Silver Ion

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Author(s)

    • Urata Shoma
    • Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University
    • Miyahata Takaaki
    • Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University
    • Matsuura Hirotaka
    • Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University
    • Kitamura Yusuke
    • Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University|CREST, Japan Science and Technology Agency
    • Ihara Toshihiro
    • Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University|CREST, Japan Science and Technology Agency

Abstract

The thermal stability of the parallel motif triple helix DNA containing CG.CH<sup>+</sup> base triplets is improved by silver ions. The N3 protons of cytosines on the third strand are replaced by the ions to form CG.CAg<sup>+</sup>, which are stable regardless of the pH values. The activity of the DNAzyme that binds its substrate DNA through triple helix formation was examined in the presence of silver ions. The DNAzyme activity was retained even at neutral pH with the assistance of silver ions.

Journal

  • Chemistry Letters

    Chemistry Letters 43(7), 1020-1022, 2014

    The Chemical Society of Japan

Codes

  • NII Article ID (NAID)
    130004868237
  • Text Lang
    ENG
  • ISSN
    0366-7022
  • Data Source
    J-STAGE 
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