Anti-inflammatory Effect of Cinnamaldehyde in <i>Helicobacter pylori</i> Induced Gastric Inflammation
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<i>Cinnamomum cassia</i> is widely employed for gastrointestinal complaints such as dyspepsia, flatulence, diarrhea, and vomiting. Studies report cinnamaldehyde (CM) as a major active constituent of cinnamon. The aim of this study was to evaluate the anti-inflammatory mechanism of CM on <i>Helicobacter</i> (<i>H.</i>) <i>pylori</i>-infected gastric epithelial cells in order to validate cinnamon traditional use in gastrointestinal (GI)-related disorders. AGS/MKN-45 cells and <i>H. pylori</i> (193C) were employed for co-culture experiments. Anti-<i>H. pylori</i> cytotoxic and anti-adhesion activity of CM were determined. Enzyme linked immunosorbent assay, real time polymerase chain reaction analysis and immunoblotting were used to measure the effect on interleukin-8 (IL-8) secretion/expression. The effect on activation of nuclear factor kappa B (NF-κB) was determined by immunoblot analysis. The non-cytotoxic CM (≤125 µM) was also non-bactericidal at the given time, suggesting the effect in <i>H</i>. <i>pylori</i>/cell co-culture system was not due to alteration in <i>H</i>. <i>pylori</i> viability or the toxicity to the cells. Also, CM did not show any anti-adhesion effect against <i>H. pylori/</i>cell co-culture. However, pre-incubation of the cells with CM significantly inhibited the IL-8 secretion/expression from <i>H. pylori</i>-infected cells (<i>p</i><0.01). In addition, CM suppressed <i>H. pylori</i>-induced NF-κB activation and prevented degradation of inhibitor (I)-κB This study provides evidence that the anti-inflammatory effect of <i>C. cassia</i> on <i>H. pylori</i>-infected gastric cells is due to blockage of the NF-κB pathway by cinnamaldehyde. This agent can be considered as a potential candidate for <i>in vivo</i> and clinical studies against various <i>H. pylori</i> related gastric pathogenic processes.
- Biological and Pharmaceutical Bulletin
Biological and Pharmaceutical Bulletin 38(1), 109-115, 2015
The Pharmaceutical Society of Japan