Highlighted Paper selected by Editor-in-Chief : Generation of Rat Induced Pluripotent Stem Cells Using a Plasmid Vector and Possible Application of a Keratan Sulfate Glycan Recognizing Antibody in Discriminating Teratoma Formation Phenotypes

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  • Makanga Juliet O.
    Laboratory of Functional Genomics, College of Pharmaceutical Sciences, Ritsumeikan University
  • Kobayashi Misa
    Laboratory of Functional Genomics, College of Pharmaceutical Sciences, Ritsumeikan University
  • Ikeda Hiroki
    Laboratory of Functional Genomics, College of Pharmaceutical Sciences, Ritsumeikan University
  • Christianto Antonius
    Laboratory of Functional Genomics, College of Pharmaceutical Sciences, Ritsumeikan University
  • Toyoda Hidenao
    Laboratory of Bio-analytical Chemistry, College of Pharmaceutical Sciences, Ritsumeikan University
  • Yamada Mitsunori
    Laboratory of Neuropathology, Department of Clinical Research, Saigata Medical Center, NHO
  • Kawasaki Toshisuke
    Research Center for Glycobiotechnology,Ritsumeikan University
  • Inazu Tetsuya
    Laboratory of Functional Genomics, College of Pharmaceutical Sciences, Ritsumeikan University

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  • Generation of Rat Induced Pluripotent Stem Cells Using a Plasmid Vector and Possible Application of a Keratan Sulfate Glycan Recognizing Antibody in Discriminating Teratoma Formation Phenotypes

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Induced pluripotent stem cells (iPSCs) offer an invaluable tool for biological research and regenerative medicine. We report establishment of rat iPSCs (riPSCs) using a plasmid vector encoding four transcription factors, Oct3/4, Sox2, c-Myc and Klf4. Although all riPSC clones were generated and cultured under the same conditions, expressed hallmark pluripotency markers and differentiated successfully in vitro, the expression of a keratan sulfate glycan epitope with unique properties defined by R-10G antibody varied in the riPSC clones. In contrast, tumor rejection antigen (TRA)-1-81 epitope expression was comparable. A clone highly reactive to R-10G antibody formed teratomas in vivo consisting of cells from all three germ layers. However, clones expressing a lower level of the epitope defined by R-10G resulted in tumors with rapid growth consisting of undifferentiated cells. Additionally, riPSCs could be successfully differentiated into a neuronal lineage including glutamate neurons that responded to agonist stimulation. These observations demonstrate a glycophenotypic difference that may potentially serve as a useful probe for riPSC evaluation and to study the role of glycans in pluripotency and carcinogenesis in these cells.

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