Pharmacological Discrimination of Plasmalemmal and Mitochondrial Sodium–Calcium Exchanger in Cardiomyocyte-Derived H9c2 Cells

• Namekata Iyuki

  Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences

• Hamaguchi Shogo

  Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences

• Tanaka Hikaru

  Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences

抄録

We examined the effects of SEA0400 and CGP-37157 on the plasmalemmal Na⁺–Ca²⁺ exchanger (NCX) and mitochondrial NCX using H9c2 cardiomyocytes loaded with Ca²⁺-sensitive fluorescent probes. The plasmalemmal NCX activity, which was measured as the increase in cytoplasmic Ca²⁺ concentration after application of low Na⁺ extracellular solution, was inhibited by SEA0400 but not by CGP-37157. The mitochondrial NCX activity, which was measured in permeabilized H9c2 cells as the decrease in mitochondrial Ca²⁺ concentration after application of Ca²⁺-free extramitochondrial solution, was inhibited by CGP-37157 but not by SEA0400. These results indicate that SEA0400 and CGP-37157 act as selective inhibitors towards plasmalemmal and mitochondrial NCX, respectively, and provide pharmacological evidence that the plasmalemmal and mitochondrial NCX are distinct molecular entities.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 38 (1), 147-150, 2015

  公益社団法人 日本薬学会

キーワード

• mitochondria
• Na⁺–Ca²⁺ exchanger
• SEA0400
• Ca²⁺ imaging

詳細情報

• CRID: 1390001204631233280
• NII論文ID: 130004872234
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b14-00525
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 026001230
• PubMed: 25744471
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026001230
• Web Site: https://www.jstage.jst.go.jp/article/bpb/38/1/38_b14-00525/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015261414
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可