Proteome Analysis of Bronchoalveolar Lavage Fluid in Chronic Hypersensitivity Pneumonitis
-
- Okamoto Tsukasa
- Department of Integrated Pulmonology, Tokyo Medical and Dental University
-
- Miyazaki Yasunari
- Department of Integrated Pulmonology, Tokyo Medical and Dental University
-
- Shirahama Ryutaro
- Department of Integrated Pulmonology, Tokyo Medical and Dental University
-
- Tamaoka Meiyo
- Department of Integrated Pulmonology, Tokyo Medical and Dental University
-
- Inase Naohiko
- Department of Integrated Pulmonology, Tokyo Medical and Dental University
この論文をさがす
抄録
Background: Hypersensitivity pneumonitis (HP) is an immune-mediated lung disease induced by inhalation of numerous antigens. Pathologically, chronic HP tends to show usual interstitial pneumonia (UIP) and fibrotic nonspecific interstitial pneumonia (fNSIP) patterns. Patients with UIP pattern present insidious onset and a risk for acute exacerbations.<br> Methods: To evaluate the proteomic differences of bronchoalveolar lavage fluid (BALF) between UIP and fNSIP patterns, BALF from seven patients with UIP pattern and four patients with fNSIP pattern was examined using two-dimensional gel electrophoresis and mass spectrometry.<br> Results: By individually comparing each BALF sample, we found that the protein levels of surfactant protein A (SP-A), immunoglobulin heavy chain α, α-2 heat shock glycoprotein, haptoglobin β, and immunoglobulin J chain were significantly higher in the patients with UIP pattern than those in the patients with fNSIP pattern. In contrast, the protein levels of glutathione s-transferase, vitamin D-binding protein, and β-actin were significantly higher in the patients with fNSIP pattern than those in the patients with UIP pattern. To confirm the results of SP-A in the BALF proteome, we performed enzyme-linked immunosorbent assay in a larger group. The concentrations of SP-A in BALF from the patients with UIP pattern were significantly higher than those from the patients with fNSIP pattern (2.331 ± 1.656 μg/ml vs. 1.319 ± 1.916 μg/ml, p = 0.034).<br> Conclusions: We identified several proteins that may play roles in the development of pathological differences between UIP and fNSIP patterns of chronic HP.<br>
収録刊行物
-
- Allergology International
-
Allergology International 61 (1), 83-92, 2012
一般社団法人日本アレルギー学会
